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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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2044 Results
  • Tertiapin-Q-ATTO Fluor-488
    Bioassay Tested
    Tertiapin-Q-ATTO Fluor-488
    A fluorescent probe for imaging and sensitive detection of Kir1.1 and GIRK potassium channels
    • Product Code: STT-170-AG
    • Short Description: A fluorescent probe for imaging and sensitive detection of Kir1.1 and GIRK potassium channels
    • Label: ATTO 488
    • Applications: Electrophysiology, Direct flow cytometry, Live cell imaging, Immunofluorescence
    • MW: 3195.5 Da
  • Stichodactyla Toxin-Biotin
    Bioassay Tested
    Stichodactyla Toxin-Biotin
    A biotinylated probe for imaging and sensitive detection of multiple KV1 channels (KV1.1, KV1.3, KV1.4, KV1.6) and KCa3.1 channels.
    • Product Code: STS-400-B
    • Short Description: A biotinylated probe for imaging and sensitive detection of multiple KV1 channels (KV1.1, KV1.3, KV1.4, KV1.6) and KCa3.1 channels.
    • Label: Biotin
    • Applications: Electrophysiology, Immunofluorescence, Indirect flow cytometry
    • MW: 4563 Da
  • Stichodactyla Toxin-ATTO Fluor-590
    Bioassay Tested
    Stichodactyla Toxin-ATTO Fluor-590
    A fluorescent probe for imaging and sensitive detection of multiple KV1 channels (KV1.1, KV1.3, KV1.4, KV1.6) and KCa3.1 channels.
    • Product Code: STS-400-AR
    • Short Description: A fluorescent probe for imaging and sensitive detection of multiple KV1 channels (KV1.1, KV1.3, KV1.4, KV1.6) and KCa3.1 channels.
    • Label: ATTO 590
    • Applications: Electrophysiology, Direct flow cytometry, Live cell imaging, Immunofluorescence
    • MW: ~4627 Da
  • MmTx1 Toxin-ATTO Fluor-488
    Bioassay Tested
    MmTx1 Toxin-ATTO Fluor-488
    A fluorescent probe for imaging and sensitive detection of GABA(A) receptors
    • Product Code: STM-550-AG
    • Short Description: A fluorescent probe for imaging and sensitive detection of GABA(A) receptors
    • Label: ATTO 488
    • Applications: Electrophysiology, Direct flow cytometry, Live cell imaging, Immunofluorescence
    • MW: ~7775 Da
  • Apamin-Biotin
    Bioassay Tested
    Apamin-Biotin
    A biotinylated apamin for imaging and sensitive detection of KCa2 potassium channels (SK channels)
    • Product Code: STA-200-B
    • Short Description: A biotinylated apamin for imaging and sensitive detection of KCa2 potassium channels (SK channels)
    • Label: Biotin
    • Applications: Electrophysiology, Immunofluorescence
    • MW: 2367 Da
  • Agitoxin-2-Cys-TAMRA
    Bioassay Tested
    Agitoxin-2-Cys-TAMRA
    A fluorescent probe for imaging and sensitive detection of KV1.3, KV1.1, and KV1.6 voltage-gated potassium channels
    • Product Code: RTA-420-T
    • Short Description: A fluorescent probe for imaging and sensitive detection of KV1.3, KV1.1, and KV1.6 voltage-gated potassium channels
    • Label: TAMRA
    • Applications: Electrophysiology, Fluorescence staining
    • MW: 4673 Da
  • α-Conotoxin ImI-ATTO Fluor-590
    Bioassay Tested
    α-Conotoxin ImI-ATTO Fluor-590
    A fluorescent probe for imaging and sensitive detection of α3β2 and α7 nicotinic acetylcholine receptors (nAChR)
    • Product Code: C-290-AR
    • Short Description: A fluorescent probe for imaging and sensitive detection of α3β2 and α7 nicotinic acetylcholine receptors (nAChR)
    • Label: ATTO 590
    • Applications: Electrophysiology, Fluorescence staining, Live cell imaging, Immunofluorescence
    • MW: 1924 Da
  • ZIP8/SLC39A8 (extracellular) Blocking Peptide
    ZIP8/SLC39A8 (extracellular) Blocking Peptide
    A Blocking Peptide for Anti-ZIP8/SLC39A8 (extracellular) Antibody
    • Product Code: BLP-ZT008
    • Short Description: A Blocking Peptide for Anti-ZIP8/SLC39A8 (extracellular) Antibody
    • Applications: Western blot, Immunohistochemistry
    • Target: SLC39A8
  • Human ZACN (extracellular) Blocking Peptide
    Human ZACN (extracellular) Blocking Peptide
    A Blocking Peptide for Anti-Human ZACN (extracellular) Antibody
    • Product Code: BLP-ZC001
    • Short Description: A Blocking Peptide for Anti-Human ZACN (extracellular) Antibody
    • Applications: Western blot, Immunohistochemistry
    • Target: ZACN
  • Oxytocin Receptor Blocking Peptide
    Oxytocin Receptor Blocking Peptide
    A Blocking Peptide for Anti-Oxytocin Receptor Antibody
    • Product Code: BLP-VR013
    • Short Description: A Blocking Peptide for Anti-Oxytocin Receptor Antibody
    • Applications: Western blot, Immunohistochemistry
    • Target: OXTR
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