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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Agitoxin-1
    Bioassay Tested
    Agitoxin-1
    A potent blocker of KV1.3, KV1.1, and KV1.6 voltage-gated potassium channels
    • Product Code: STA-150
    • Short Description: A potent blocker of KV1.3, KV1.1, and KV1.6 voltage-gated potassium channels
    • Applications: Electrophysiology
    • CAS No.: 155646-21-2
    • MW: 4015 Da
  • Aam-KTX
    Bioassay Tested
    Aam-KTX
    A blocker of voltage-gated potassium (KV) channels, particularly KV1.3
    • Product Code: STA-110
    • Short Description: A blocker of voltage-gated potassium (KV) channels, particularly KV1.3
    • Applications: Electrophysiology
    • MW: 4185 Da
  • Azemiopsin
    Bioassay Tested
    Azemiopsin
    An antagonist of muscle α1/β1/γ/δ nicotinic acetylcholine receptors (nAChR)
    • Product Code: STA-100
    • Short Description: An antagonist of muscle α1/β1/γ/δ nicotinic acetylcholine receptors (nAChR)
    • Applications: Electrophysiology
    • CAS No.: 1391936-86-9
    • MW: 2541 Da
  • human GDNF proDomain
    human GDNF proDomain
    A synthetic human GDNF prodomain, primarily used for studying the intracellular trafficking and regulated secretion of mature GDNF via the SorLA receptor
    • Product Code: SPG-100
    • Short Description: A synthetic human GDNF prodomain, primarily used for studying the intracellular trafficking and regulated secretion of mature GDNF via the SorLA receptor
    • Applications: Cell-Based Assays, Western blot
    • MW: 6334 Da
  • TAK-044
    Bioassay Tested
    TAK-044
    A potent endothelin receptor antagonist that targets both ETA and ETB subtypes, showing a minor preference for ETA
    • Product Code: SPE-320
    • Short Description: A potent endothelin receptor antagonist that targets both ETA and ETB subtypes, showing a minor preference for ETA
    • Applications: ETB assay, Calcium imaging assay
    • CAS No.: 157380-72-8
    • MW: 927 Da
  • [Ala<sup>1,3,11,15</sup>] Endothelin-1
    Bioassay Tested
    [Ala1,3,11,15] Endothelin-1
    A potent and selective agonist for the endothelin B (ETB) receptors
    • Product Code: SPE-240
    • Short Description: A potent and selective agonist for the endothelin B (ETB) receptors
    • Applications: Aequorin functional assay, Calcium imaging assay
    • CAS No.: 121204-87-3
    • MW: 2367.7 Da
  • IRL-1620
    Bioassay Tested
    IRL-1620
    A potent and selective agonist for the endothelin B (ETB) receptors
    • Product Code: SPE-220
    • Short Description: A potent and selective agonist for the endothelin B (ETB) receptors
    • Applications: Aequorin functional assay, Calcium imaging assay
    • CAS No.: 142569-99-1
    • MW: 1820 Da
  • BQ-3020
    Bioassay Tested
    BQ-3020
    A potent and selective agonist for the endothelin B (ETB) receptors
    • Product Code: SPE-200
    • Short Description: A potent and selective agonist for the endothelin B (ETB) receptors
    • Applications: Aequorin functional assay, Calcium imaging assay
    • CAS No.: 143113-45-5
    • MW: 2006 Da
  • Sarafotoxin S6b
    Bioassay Tested
    Sarafotoxin S6b
    A potent, non-selective agonist for ETA and ETB endothelin receptors
    • Product Code: SPE-160
    • Short Description: A potent, non-selective agonist for ETA and ETB endothelin receptors
    • Applications: Aequorin functional assay, Calcium imaging assay
    • CAS No.: 116303-65-2
    • MW: 2564 Da
  • Calcicludine
    Bioassay Tested
    Calcicludine
    A non-selective blocker of multiple high-voltage-activated calcium channels, including L-type (CaV1.2, 1.3), P-type (CaV2.1), and N-type (CaV2.2) channels
    • Product Code: SPC-650
    • Short Description: A non-selective blocker of multiple high-voltage-activated calcium channels, including L-type (CaV1.2, 1.3), P-type (CaV2.1), and N-type (CaV2.2) channels
    • Applications: Electrophysiology
    • CAS No.: 178037-96-2
    • MW: 6980 Da
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