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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • ICA 121431
    Bioassay Tested
    ICA 121431
    A Potent and Selective Blocker of NaV1.3 and NaV1.1 Channels
    • Product Code: I-170
    • Short Description: A Potent and Selective Blocker of NaV1.3 and NaV1.1 Channels
    • CAS No.: 313254-51-2
    • MW: 449.5
  • Icilin
    Bioassay Tested
    Icilin
    A Potent Activator of TRPM8 Channels
    • Product Code: I-145
    • Short Description: A Potent Activator of TRPM8 Channels
    • CAS No.: 36945-98-9
    • MW: 311.29
  • HA-1077
    Bioassay Tested
    HA-1077
    A Rho/Rok Inhibitor
    • Product Code: H-220
    • Short Description: A Rho/Rok Inhibitor
    • CAS No.: 103745-39-7
    • MW: 364.3
  • HMA
    Bioassay Tested
    HMA
    An Antagonist of Na+/H+ Exchangers, P2X7 receptors and TRPA1, ASIC3 Channels
    • Product Code: H-130
    • Short Description: An Antagonist of Na+/H+ Exchangers, P2X7 receptors and TRPA1, ASIC3 Channels
    • CAS No.: 1428-95-1
    • MW: 311.77
  • HC-030031
    Bioassay Tested
    HC-030031
    A Selective TRPA1 Channel Blocker
    • Product Code: H-105
    • Short Description: A Selective TRPA1 Channel Blocker
    • CAS No.: 349085-38-7
    • MW: 355.4
  • WRW4
    Bioassay Tested
    WRW4
    A potent antagonist selective for the FPR2 and FPR3 G-protein coupled receptors
    • Product Code: GPW-110
    • Short Description: A potent antagonist selective for the FPR2 and FPR3 G-protein coupled receptors
    • Applications: Calcium imaging assay
    • CAS No.: 878557-55-2
    • MW: 1104.3 Da
  • WKYMVm
    Bioassay Tested
    WKYMVm
    A potent, non-selective agonist for formyl peptide G-protein coupled receptors (FPR1, FPR2, and FPR3)
    • Product Code: GPW-105
    • Short Description: A potent, non-selective agonist for formyl peptide G-protein coupled receptors (FPR1, FPR2, and FPR3)
    • Applications: Calcium imaging assay
    • CAS No.: 187986-17-0
    • MW: 856.11 Da
  • WKYMVM
    Bioassay Tested
    WKYMVM
    A potent agonist with high selectivity for FPR2 G-protein coupled receptors, and a less potent agonist for the FPR3 receptors
    • Product Code: GPW-100
    • Short Description: A potent agonist with high selectivity for FPR2 G-protein coupled receptors, and a less potent agonist for the FPR3 receptors
    • Applications: Calcium imaging assay
    • CAS No.: 187986-11-4
    • MW: 856.11 Da
  • SLIGKV-NH2
    Bioassay Tested
    SLIGKV-NH2
    A potent agonist of the PAR2 G-protein coupled receptors
    • Product Code: GPS-100
    • Short Description: A potent agonist of the PAR2 G-protein coupled receptors
    • Applications: Aequorin functional assay, Western blot, Calcium imaging assay
    • CAS No.: 190383-13-2
    • MW: 614.77 Da
  • Neurotensin
    Bioassay Tested
    Neurotensin
    A potent non-selective agonist of neurotensin receptors (NTSR1, NTSR2 and SORT1)
    • Product Code: GPN-100
    • Short Description: A potent non-selective agonist of neurotensin receptors (NTSR1, NTSR2 and SORT1)
    • Applications: Calcium imaging assay
    • CAS No.: 55508-42-4
    • MW: 1673 Da
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