Inflammation

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • MMK1
    Bioassay Tested
    MMK1
    A potent agonist selective for the FPR2 G-Protein Coupled Receptors
    • Product Code: GPM-120
    • Short Description: A potent agonist selective for the FPR2 G-Protein Coupled Receptors
    • Applications: Calcium imaging assay
    • CAS No.: 271246-66-3
    • MW: 1611 Da
  • α-MSH
    Bioassay Tested
    α-MSH
    A potent agonist for melanocortin receptors, primarily involved in pigmentation, inflammation control, and appetite regulation
    • Product Code: GPM-100
    • Short Description: A potent agonist for melanocortin receptors, primarily involved in pigmentation, inflammation control, and appetite regulation
    • Applications: Melanin production assay
    • CAS No.: 581-05-5
    • MW: 1665 Da
  • Lys-Bradykinin
    Bioassay Tested
    Lys-Bradykinin
    A potent agonist of the B2 bradykinin G-protein coupled receptors
    • Product Code: GPK-100
    • Short Description: A potent agonist of the B2 bradykinin G-protein coupled receptors
    • Applications: Calcium imaging assay
    • CAS No.: 0342-10-9
    • MW: 1188.38 Da
  • N-Formyl-Met-Leu-Phe
    Bioassay Tested
    N-Formyl-Met-Leu-Phe
    A potent agonist of FPR1 G-protein coupled receptor
    • Product Code: GPF-100
    • Short Description: A potent agonist of FPR1 G-protein coupled receptor
    • Applications: Calcium imaging assay
    • CAS No.: 59880-97-6
    • MW: 437.6 Da
  • Contulakin-G
    Bioassay Tested
    Contulakin-G
    A synthetic, non-glycosylated neurotensin analog and potent agonist for the neurotensin receptors (NTSR1, NTSR2 and SORT1)
    • Product Code: GPC-100
    • Short Description: A synthetic, non-glycosylated neurotensin analog and potent agonist for the neurotensin receptors (NTSR1, NTSR2 and SORT1)
    • Applications: Calcium imaging assay
    • CAS No.: 229180-41-0
    • MW: 1704.8 Da
  • Bradykinin
    Bioassay Tested
    Bradykinin
    A potent agonist selective for the B2 bradykinin G-protein coupled receptor
    • Product Code: GPB-200
    • Short Description: A potent agonist selective for the B2 bradykinin G-protein coupled receptor
    • Applications: Calcium imaging assay
    • CAS No.: 58-82-2
    • MW: 1060.23 Da
  • Bombesin
    Bioassay Tested
    Bombesin
    A potent agonist for the Bombesin (BB1, BB2, and BB3) G-protein coupled receptors
    • Product Code: GPB-100
    • Short Description: A potent agonist for the Bombesin (BB1, BB2, and BB3) G-protein coupled receptors
    • Applications: Calcium imaging assay
    • CAS No.: 31362-50-2
    • MW: 1620.8 Da
  • Angiotensin III
    Bioassay Tested
    Angiotensin III
    A potent agonist for AT1 and AT2 G-protein coupled receptors
    • Product Code: GPA-130
    • Short Description: A potent agonist for AT1 and AT2 G-protein coupled receptors
    • Applications: Aequorin functional assay, Western blot, Calcium imaging assay
    • CAS No.: 13602-53-4
    • MW: 931.1 Da
  • Angiotensin II
    Bioassay Tested
    Angiotensin II
    A potent agonist for AT1 and AT2 G-protein coupled receptors
    • Product Code: GPA-100
    • Short Description: A potent agonist for AT1 and AT2 G-protein coupled receptors
    • Applications: Aequorin functional assay, Western blot, Calcium imaging assay
    • CAS No.: 4474-91-3
    • MW: 1046.2 Da
  • Geldanamycin
    Bioassay Tested
    Geldanamycin
    A Powerful Anticancer Antibiotic
    • Product Code: G-500
    • Short Description: A Powerful Anticancer Antibiotic
    • CAS No.: 30562-34-6
    • MW: 560.6
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