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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2045 Results
  • (+)-Bicuculline
    Bioassay Tested
    (+)-Bicuculline
    A Potent Antagonist of GABA(A) Receptors
    • Product Code: B-135
    • Short Description: A Potent Antagonist of GABA(A) Receptors
    • CAS No.: 485-49-4
    • MW: 367.4
  • BCTC
    Bioassay Tested
    BCTC
    A Potent Antagonist of TRPV1 and TRPM8 Channels
    • Product Code: B-110
    • Short Description: A Potent Antagonist of TRPV1 and TRPM8 Channels
    • CAS No.: 393514-24-4
    • MW: 372.9
  • α-Bungarotoxin-ATTO Fluor-633
    Bioassay Tested
    α-Bungarotoxin-ATTO Fluor-633
    A fluorescent probe for imaging and sensitive detection of neuromuscular junctions, GABA(A) receptors, nAChRs, and α-Bungarotoxin binding sites
    • Product Code: B-100-FR
    • Short Description: A fluorescent probe for imaging and sensitive detection of neuromuscular junctions, GABA(A) receptors, nAChRs, and α-Bungarotoxin binding sites
    • Label: ATTO 633
    • Applications: Electrophysiology, Direct flow cytometry, Fluorescence staining, Live cell imaging, Immunofluorescence
    • MW: ~9140 Da
  • α-Bungarotoxin-ATTO Fluor-488
    Bioassay Tested
    α-Bungarotoxin-ATTO Fluor-488
    A fluorescent probe for imaging and sensitive detection of neuromuscular junctions, GABA(A) receptors, nAChRs, and α-Bungarotoxin binding sites
    • Product Code: B-100-AG
    • Short Description: A fluorescent probe for imaging and sensitive detection of neuromuscular junctions, GABA(A) receptors, nAChRs, and α-Bungarotoxin binding sites
    • Label: ATTO 488
    • Applications: Electrophysiology, Direct flow cytometry, Fluorescence staining
    • MW: ~9140 Da
  • Anti-ZIP10/SLC39A10 (extracellular)-FITC Antibody
    Anti-ZIP10/SLC39A10 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to ZIP10/SLC39A10 conjugated to fluorescein isothiocyanate (FITC) fluorophore
    • Product Code: AZT-010-F
    • Short Description: A Rabbit Polyclonal Antibody to ZIP10/SLC39A10 conjugated to fluorescein isothiocyanate (FITC) fluorophore
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Zinc transporter ZIP10, Zrt- and Irt-like protein 10, Solute Carrier Family 39 Member 10
  • Anti-ZIP10/SLC39A10 (extracellular) Antibody
    Anti-ZIP10/SLC39A10 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to ZIP10/SLC39A10 (extracellular)
    • Product Code: AZT-010
    • Short Description: A Rabbit Polyclonal Antibody to ZIP10/SLC39A10 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Zinc transporter ZIP10, Zrt- and Irt-like protein 10, Solute Carrier Family 39 Member 10
  • Anti-ZIP8 (SLC39A8) (extracellular)-FITC Antibody
    Anti-ZIP8 (SLC39A8) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Zinc Transporter ZIP8 Conjugated to the Fluorescent Dye FITC
    • Product Code: AZT-008-F
    • Short Description: A Rabbit Polyclonal Antibody to Zinc Transporter ZIP8 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: Zinc transporter ZIP8, Zrt- and Irt-like protein 8, Solute carrier family 39 member 8, BIGM103
  • Anti-ZIP8 (SLC39A8) (extracellular) Antibody
    Anti-ZIP8 (SLC39A8) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Zinc Transporter ZIP8
    • Product Code: AZT-008
    • Short Description: A Rabbit Polyclonal Antibody to Zinc Transporter ZIP8
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Zinc transporter ZIP8, Zrt- and Irt-like protein 8, Solute carrier family 39 member 8, BIGM103
  • Anti-Human ZACN (extracellular) Antibody
    Anti-Human ZACN (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Zinc-Activated Ion Channel
    • Product Code: AZC-001
    • Short Description: A Rabbit Polyclonal Antibody to Zinc-Activated Ion Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: Zinc-activated ligand-gated ion channel, Ligand-gated ion channel zinc-activated 1, Ligand-gated ion-channel receptor L2, ZAC, LGICZ1
  • Anti-Oxytocin Receptor Antibody
    KO Validate'
    Anti-Oxytocin Receptor Antibody
    A Rabbit Polyclonal Antibody to Oxytocin Receptor
    • Product Code: AVR-013
    • Short Description: A Rabbit Polyclonal Antibody to Oxytocin Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: OXTR, OT-R, OTR1
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