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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2045 Results
  • Anti-Semaphorin 3A (SEMA3A) Antibody
    Anti-Semaphorin 3A (SEMA3A) Antibody
    A Rabbit Polyclonal Antibody to Semaphorin 3A
    • Product Code: ASR-051
    • Short Description: A Rabbit Polyclonal Antibody to Semaphorin 3A
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Semaphorin III, Collapsin 1
  • Anti-5HT7 Receptor/HTR7 (extracellular)-FITC Antibody
    Anti-5HT7 Receptor/HTR7 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to 5HT7 receptor (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ASR-037-F
    • Short Description: A Rabbit Polyclonal Antibody to 5HT7 receptor (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: 5-Hydroxytryptamine Receptor 7, Serotonin Receptor 7, 5-HT7, 5-HT-7
  • Anti-5HT7 Receptor/HTR7 (extracellular) Antibody
    Anti-5HT7 Receptor/HTR7 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to 5HT7 Receptor
    • Product Code: ASR-037
    • Short Description: A Rabbit Polyclonal Antibody to 5HT7 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: 5-Hydroxytryptamine Receptor 7, Serotonin Receptor 7, 5-HT7, 5-HT-7
  • Anti-5HT4 Receptor (HTR4) (extracellular)-FITC Antibody
    Anti-5HT4 Receptor (HTR4) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 4 Conjugated to the Fluorescent Dye FITC
    • Product Code: ASR-036-F
    • Short Description: A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 4 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: 5-Hydroxytryptamine receptor 4, Serotonin receptor 4
  • Anti-5HT4 Receptor (HTR4) (extracellular) Antibody
    KO Validate'
    Anti-5HT4 Receptor (HTR4) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 4
    • Product Code: ASR-036
    • Short Description: A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 4
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry
    • Target: 5-Hydroxytryptamine receptor 4, Serotonin receptor 4
  • Anti-5HT2B Receptor (HTR2B) (extracellular)-ATTO Fluor-488 Antibody
    Anti-5HT2B Receptor (HTR2B) (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 2B Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: ASR-035-AG
    • Short Description: A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 2B Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry, Immunohistochemistry
    • Target: 5-Hydroxytryptamine receptor 2B, Serotonin receptor 2B, 5-HT-2B, 5-HT2F, Stomach fundus serotonin receptor
  • Anti-5HT2B Receptor (HTR2B) (extracellular) Antibody
    Anti-5HT2B Receptor (HTR2B) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 2B
    • Product Code: ASR-035
    • Short Description: A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 2B
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: 5-Hydroxytryptamine receptor 2B, Serotonin receptor 2B, 5-HT-2B, 5-HT2F, Stomach fundus serotonin receptor
  • Anti-5HT2C Receptor (HTR2C) Antibody
    Anti-5HT2C Receptor (HTR2C) Antibody
    A Rabbit Polyclonal antibody to 5HT2C Receptor (HTR2C)
    • Product Code: ASR-034
    • Short Description: A Rabbit Polyclonal antibody to 5HT2C Receptor (HTR2C)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: 5-Hydroxytryptamine receptor 2C, Serotonin receptor 2C, 5-HT2C
  • Guinea Pig Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody
    Guinea Pig Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody
    A Guinea Pig Polyclonal Antibody to 5-HT2A Receptor
    • Product Code: ASR-033-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to 5-HT2A Receptor
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: 5-Hydroxytryptamine receptor 2A, Serotonin receptor 2A, 5-HT2A
  • Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody
    Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 2A
    • Product Code: ASR-033
    • Short Description: A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 2A
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunohistochemistry
    • Target: 5-Hydroxytryptamine receptor 2A, Serotonin receptor 2A
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