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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Anti-SCN3A (Na<sub>V</sub>1.3) (extracellular) Antibody
    Anti-SCN3A (NaV1.3) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to SCN3A (NaV1.3) Channel
    • Product Code: ASC-023
    • Short Description: A Rabbit Polyclonal Antibody to SCN3A (NaV1.3) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: BIII, Brain type III Na+ channel, Sodium channel protein type 3 subunit alpha
  • Anti-SCN4A (Na<sub>V</sub>1.4) Antibody
    Anti-SCN4A (NaV1.4) Antibody
    A Rabbit Polyclonal Antibody to SCN4A (NaV1.4) Channel
    • Product Code: ASC-020
    • Short Description: A Rabbit Polyclonal Antibody to SCN4A (NaV1.4) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence
    • Target: Sodium channel protein type 4 subunit α, SkM1, Sodium channel protein skeletal muscle subunit α, HYKPP
  • Anti-ENaC β (SCNN1B) (extracellular) Antibody
    Anti-ENaC β (SCNN1B) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to ENaCβ Channel
    • Product Code: ASC-019
    • Short Description: A Rabbit Polyclonal Antibody to ENaCβ Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Amiloride-sensitive sodium channel subunit beta, Beta-NaCH, Epithelial sodium channel β subunit, Beta-ENaC, Nonvoltage-gated sodium channel 1 subunit beta, SCNEB
  • Guinea pig Anti-ASIC3 Antibody
    Guinea pig Anti-ASIC3 Antibody
    A Guinea Pig Polyclonal Antibody to ASIC3 Channel
    • Product Code: ASC-018-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to ASIC3 Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Acid-sensing ion channel 3, Amiloride-sensitive cation channel 3, ACCN3, DRASIC
  • Anti-ASIC3 Antibody
    KO Validate'
    Anti-ASIC3 Antibody
    A Rabbit Polyclonal Antibody to ASIC3 Channel
    • Product Code: ASC-018
    • Short Description: A Rabbit Polyclonal Antibody to ASIC3 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Acid-sensing ion channel 3, Amiloride-sensitive cation channel 3, ACCN3, DRASIC
  • Guinea Pig Anti-SCN11A (Na<sub>V</sub>1.9) Antibody
    Guinea Pig Anti-SCN11A (NaV1.9) Antibody
    A Guinea Pig Polyclonal Antibody to SCN11A (NaV1.9) Channel
    • Product Code: ASC-017-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to SCN11A (NaV1.9) Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: PN5, SNS2, NAN, Sodium channel protein type 11 subunit alpha
  • Anti-SCN11A (Na<sub>V</sub>1.9) Antibody
    Anti-SCN11A (NaV1.9) Antibody
    A Rabbit Polyclonal Antibody to SCN11A (NaV1.9) Channel
    • Product Code: ASC-017
    • Short Description: A Rabbit Polyclonal Antibody to SCN11A (NaV1.9) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: PN5, SNS2, NAN, Sodium channel protein type 11 subunit alpha
  • Guinea pig Anti-Na<sub>V</sub>1.8 (SCN10A) Antibody
    Guinea pig Anti-NaV1.8 (SCN10A) Antibody
    A Guinea Pig Polyclonal Antibody to NaV1.8 (SCN10A) Channel
    • Product Code: ASC-016-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to NaV1.8 (SCN10A) Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: PN3, SNS, Sodium channel protein type 10 subunit alpha
  • Anti-Na<sub>V</sub>1.8 (SCN10A)-ATTO Fluor-594 Antibody
    Anti-NaV1.8 (SCN10A)-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to NaV1.8 (SCN10A) Channel Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ASC-016-AR
    • Short Description: A Rabbit Polyclonal Antibody to NaV1.8 (SCN10A) Channel Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: PN3, SNS, Sodium channel protein type 10 subunit alpha
  • Anti-Na<sub>V</sub>1.8 (SCN10A) Antibody
    KO Validate'
    Anti-NaV1.8 (SCN10A) Antibody
    A Rabbit Polyclonal Antibody to NaV1.8 (SCN10A) Channel
    • Product Code: ASC-016
    • Short Description: A Rabbit Polyclonal Antibody to NaV1.8 (SCN10A) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: PN3, SNS, Sodium channel protein type 10 subunit alpha
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