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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Guinea pig Anti-SCN1A (Na<sub>V</sub>1.1) Antibody
    Guinea pig Anti-SCN1A (NaV1.1) Antibody
    A Guinea Pig Polyclonal Antibody to SCN1A (NaV1.1) Channel
    • Product Code: ASC-001-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to SCN1A (NaV1.1) Channel
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Brain type I sodium channel, BI, Sodium channel protein type 1 subunit α
  • Anti-SCN1A (Na<sub>V</sub>1.1)-ATTO Fluor-594 Antibody
    KO Validate'
    Anti-SCN1A (NaV1.1)-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to SCN1A (NaV1.1) Channel Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ASC-001-AR
    • Short Description: A Rabbit Polyclonal Antibody to SCN1A (NaV1.1) Channel Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Brain type I sodium channel, BI, Sodium channel protein type 1 subunit α
  • Anti-RAMP2 (extracellular) Antibody
    Anti-RAMP2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to RAMP2
    • Product Code: ARR-022
    • Short Description: A Rabbit Polyclonal Antibody to RAMP2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: Receptor activity-modifying protein 2, CRLR activity-modifying protein 2, Calcitonin receptor-like receptor activity-modifying protein 2
  • Anti-RAMP1 (extracellular) Antibody
    KO Validate'
    Anti-RAMP1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to RAMP1
    • Product Code: ARR-021
    • Short Description: A Rabbit Polyclonal Antibody to RAMP1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Receptor activity-modifying protein 1, Calcitonin receptor-like receptor activity-modifying protein 1
  • Anti-Ryanodine Receptor 3 Antibody
    Anti-Ryanodine Receptor 3 Antibody
    A Rabbit Polyclonal Antibody to Ryanodine Receptor 3
    • Product Code: ARR-003
    • Short Description: A Rabbit Polyclonal Antibody to Ryanodine Receptor 3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: RyR3, Brain-type ryanodine receptor
  • Anti-Ryanodine Receptor 1 Antibody
    Anti-Ryanodine Receptor 1 Antibody
    A Rabbit Polyclonal Antibody to Ryanodine Receptor 1
    • Product Code: ARR-001
    • Short Description: A Rabbit Polyclonal Antibody to Ryanodine Receptor 1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: RyR1, Skeletal muscle-type ryanodine receptor
  • Guinea Pig Anti-Aquaporin 4 (AQP4) (300-314) Antibody
    Guinea Pig Anti-Aquaporin 4 (AQP4) (300-314) Antibody
    A Guinea Pig Polyclonal Antibody to Aquaporin 4 (AQP4)
    • Product Code: AQP-014-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Aquaporin 4 (AQP4)
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: WCH4, Mercurial-insensitive water channel, MIWC
  • Anti-Aquaporin 4 (AQP4) (300-314)-ATTO Fluor-594 Antibody
    Anti-Aquaporin 4 (AQP4) (300-314)-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to Aquaporin 4 Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: AQP-014-AR
    • Short Description: A Rabbit Polyclonal Antibody to Aquaporin 4 Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: WCH4, Mercurial-insensitive water channel
  • Anti-Aquaporin 4 (AQP4) (300-314) Antibody
    Anti-Aquaporin 4 (AQP4) (300-314) Antibody
    A Rabbit Polyclonal Antibody to Aquaporin 4 Channel
    • Product Code: AQP-014
    • Short Description: A Rabbit Polyclonal Antibody to Aquaporin 4 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: WCH4, Mercurial-insensitive water channel
  • Anti-Aquaporin 11 (extracellular) Antibody
    Anti-Aquaporin 11 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Aquaporin 11 Channel
    • Product Code: AQP-011
    • Short Description: A Rabbit Polyclonal Antibody to Aquaporin 11 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: AQP11
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