Inflammation

Inflammation

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.
  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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2041 Results
  • Guinea Pig Anti-Homer1 Antibody
    A Guinea Pig Polyclonal Antibody to Homer1
    • Product Code: APZ-026-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Homer1
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Homer protein homolog 1, Homer scaffolding protein 1, Vesl-1, Ves 1, PSD-Zip45, SYN47
  • Anti-Homer1 Antibody
    A Rabbit Polyclonal Antibody to Homer1
    • Product Code: APZ-026
    • Short Description: A Rabbit Polyclonal Antibody to Homer1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Homer protein homolog 1, Homer scaffolding protein 1, Vesl-1, Ves 1, PSD-Zip45, SYN47
  • Anti-GRIP1 Antibody
    A Rabbit Polyclonal Antibody to GRIP1
    • Product Code: APZ-015
    • Short Description: A Rabbit Polyclonal Antibody to GRIP1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Glutamate receptor interacting protein 1
  • Anti-PICK1 Antibody
    A Rabbit Polyclonal Antibody to PICK1
    • Product Code: APZ-014
    • Short Description: A Rabbit Polyclonal Antibody to PICK1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: PRKCA-binding protein, PRKCABP, Protein interacting with C kinase 1, Protein interacting with PRKCA1, Protein kinase C-alpha-binding protein
  • Anti-SAP-97/DLG1 Antibody
    A Rabbit Polyclonal Antibody to SAP-97
    • Product Code: APZ-010
    • Short Description: A Rabbit Polyclonal Antibody to SAP-97
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Synapse-associated protein 97, Discs large homolog 1
  • Guinea Pig Anti-PSD-95 Antibody
    A Guinea Pig Polyclonal Antibody to PSD-95
    • Product Code: APZ-009-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to PSD-95
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Postsynaptic density protein 95, Discs large homolog 4, DLG4, Synapse-associated protein 90, SAP-90, Tax interaction protein 15, MRD62
  • Anti-SAP102 Antibody
    A Rabbit Polyclonal Antibody to SAP-102
    • Product Code: APZ-003
    • Short Description: A Rabbit Polyclonal Antibody to SAP-102
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Synapse-associated protein 102, SAP-102, Discs large homolog 3, DLG3, NE-DLG
  • Anti-PSD-93 Antibody
    A Rabbit Polyclonal Antibody to PSD-93
    • Product Code: APZ-002
    • Short Description: A Rabbit Polyclonal Antibody to PSD-93
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Discs large homolog 2, Dlg2, Chapsyn-110
  • Anti-PepT2/SLC15A2 (extracellular) Antibody
    A Rabbit Polyclonal antibody to PepT2/SLC15A2 (extracellular)
    • Product Code: APT-002
    • Short Description: A Rabbit Polyclonal antibody to PepT2/SLC15A2 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Peptide Transporter 2, Solute Carrier Family 15 Member 2, Oligopeptide Transporter - Kidney Isoform, Kidney H+/Peptide Cotransporter
  • Anti-P2Y13 Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to P2Y13 Receptor  (extracellular) conjugated to the fluorescent dye FITC 
    • Product Code: APR-103-F
    • Short Description: A Rabbit Polyclonal Antibody to P2Y13 Receptor  (extracellular) conjugated to the fluorescent dye FITC 
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Purinergic Receptor P2Y13, P2RY13, GPCR1, GPR86, GPR94, SP174
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