Inflammation

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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2041 Results
  • Anti-proBDNF Antibody
    Anti-proBDNF Antibody
    A Rabbit Polyclonal Antibody to proBDNF
    • Product Code: ANT-006
    • Short Description: A Rabbit Polyclonal Antibody to proBDNF
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Brain-derived neurotrophic factor precursor
  • Guinea pig Anti-proNGF Antibody
    Guinea pig Anti-proNGF Antibody
    A Guinea Pig Polyclonal Antibody to proNGF
    • Product Code: ANT-005-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to proNGF
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Nerve growth factor precursor
  • Anti-proNGF Antibody
    Anti-proNGF Antibody
    A Rabbit Polyclonal Antibody to proNGF
    • Product Code: ANT-005
    • Short Description: A Rabbit Polyclonal Antibody to proNGF
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Nerve growth factor precursor
  • Anti-Neurotrophin 3 (NT-3) Antibody
    Anti-Neurotrophin 3 (NT-3) Antibody
    A Rabbit Polyclonal Antibody to Neurotrophin 3
    • Product Code: ANT-003
    • Short Description: A Rabbit Polyclonal Antibody to Neurotrophin 3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: NTF3, Nerve growth factor 2, NGF2, HDNF
  • Anti-Cadherin-13 (extracellular) Antibody
    Anti-Cadherin-13 (extracellular) Antibody
    A rabbit polyclonal antibody to Cadherin-13 (extracellular)
    • Product Code: ANR-233
    • Short Description: A rabbit polyclonal antibody to Cadherin-13 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: CDH13
  • Anti-SLIT1 Antibody
    Anti-SLIT1 Antibody
    A Rabbit Polyclonal antibody to SLIT1
    • Product Code: ANR-221
    • Short Description: A Rabbit Polyclonal antibody to SLIT1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Slit Guidance Ligand 1, Slit Homolog 1 Protein, Multiple EGF-Like Domains Protein 4, MEGF4
  • Anti-PTGFRN (extracellular) Antibody
    Anti-PTGFRN (extracellular) Antibody
    A Rabbit polyclonal antibody to PTGFRN (extracellular)
    • Product Code: ANR-212
    • Short Description: A Rabbit polyclonal antibody to PTGFRN (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Prostaglandin F2 Receptor Inhibitor, CD9 Partner 1, CD9P-1, FPRP, Glu-Trp-Ile EWI motif-containing protein F, EWI-F, CD315
  • Anti-CD81 (extracellular)-PE Antibody
    Anti-CD81 (extracellular)-PE Antibody
    A Rabbit Polyclonal antibody to CD81 (extracellular) conjugated to the fluorescent dye Phycoerythrin (PE).
    • Product Code: ANR-211-PE
    • Short Description: A Rabbit Polyclonal antibody to CD81 (extracellular) conjugated to the fluorescent dye Phycoerythrin (PE).
    • Host: Rabbit
    • Label: R-PE
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Tetraspanin-28, TSPAN28, Target of the Antiproliferative Antibody 1, TAPA-1
  • Anti-CD81 (extracellular)-FITC Antibody
    Anti-CD81 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to CD81 (extracellular) (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ANR-211-F
    • Short Description: A Rabbit Polyclonal Antibody to CD81 (extracellular) (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Tetraspanin-28, TSPAN28, Target of the Antiproliferative Antibody 1, TAPA-1
  • Anti-CD81 (extracellular)-APC Antibody
    Anti-CD81 (extracellular)-APC Antibody
    A Rabbit Polyclonal antibody to CD81 (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Product Code: ANR-211-APC
    • Short Description: A Rabbit Polyclonal antibody to CD81 (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Host: Rabbit
    • Label: APC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Tetraspanin-28, TSPAN28, Target of the Antiproliferative Antibody 1, TAPA-1
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