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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Anti-SEZ6 (extracellular) Antibody
    Anti-SEZ6 (extracellular) Antibody
    A Rabbit Polyclonal antibody to SEZ6
    • Product Code: ANR-206
    • Short Description: A Rabbit Polyclonal antibody to SEZ6
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Seizure Protein 6, Seizure Related 6 Homolog
  • Anti-MEGF10 (extracellular) Antibody
    Anti-MEGF10 (extracellular) Antibody
    A Rabbit Polyclonal antibody to MEGF10 (extracellular)
    • Product Code: ANR-205
    • Short Description: A Rabbit Polyclonal antibody to MEGF10 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Multiple EGF Like Domains 10, Multiple EGF-Like Domains Protein 10
  • Anti-CADM1/SynCAM (extracellular) Antibody
    Anti-CADM1/SynCAM (extracellular) Antibody
    A Rabbit Polyclonal antibody to CADM1/SynCAM (extracellular)
    • Product Code: ANR-201
    • Short Description: A Rabbit Polyclonal antibody to CADM1/SynCAM (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Cell Adhesion Molecule 1, Synaptic Cell Adhesion Molecule, Nectin-Like 2, NECL-2, Immunoglobulin Superfamily Member 4, IgSF4
  • Anti-Neogenin (extracellular)-FITC Antibody
    Anti-Neogenin (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Neogenin (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ANR-195-F
    • Short Description: A Rabbit Polyclonal Antibody to Neogenin (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Neogenin 1, NEO1, NGN, NTN1R2
  • Anti-Neogenin (extracellular) Antibody
    Anti-Neogenin (extracellular) Antibody
    A Rabbit Polyclonal antibody to Neogenin (extracellular)
    • Product Code: ANR-195
    • Short Description: A Rabbit Polyclonal antibody to Neogenin (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Neogenin 1, NEO1, NGN
  • Anti-Neuronal Pentraxin Receptor (NPTXR) (extracellular)-FITC Antibody
    Anti-Neuronal Pentraxin Receptor (NPTXR) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Neuronal Pentraxin Receptor (NPTXR) (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ANR-193-F
    • Short Description: A Rabbit Polyclonal Antibody to Neuronal Pentraxin Receptor (NPTXR) (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: NPR, NPTXR
  • Anti-Neuronal Pentraxin Receptor (NPTXR)(extracellular) Antibody
    Anti-Neuronal Pentraxin Receptor (NPTXR)(extracellular) Antibody
    A Rabbit Polyclonal Antibody to NPTXR (extracellular)
    • Product Code: ANR-193
    • Short Description: A Rabbit Polyclonal Antibody to NPTXR (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: NPR, NPTXR
  • Anti-Neuronal Pentraxin 2 (NPTX2) Antibody
    Anti-Neuronal Pentraxin 2 (NPTX2) Antibody
    A Rabbit Polyclonal antibody to Neuronal Pentraxin 2 (NPTX2)
    • Product Code: ANR-192
    • Short Description: A Rabbit Polyclonal antibody to Neuronal Pentraxin 2 (NPTX2)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Neuronal Pentraxin-2, NP2, Neuronal Activity-Regulated Pentaxin, NARP
  • Anti-Neuronal Pentraxin 1 (NPTX1) Antibody
    Anti-Neuronal Pentraxin 1 (NPTX1) Antibody
    A Rabbit Polyclonal antibody to Neuronal Pentraxin 1 (NPTX1)
    • Product Code: ANR-191
    • Short Description: A Rabbit Polyclonal antibody to Neuronal Pentraxin 1 (NPTX1)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Neuronal Pentraxin-1, NP1
  • Anti-Tetraspanin 18 (extracellular) Antibody
    Anti-Tetraspanin 18 (extracellular) Antibody
    A Rabbit Polyclonal antibody to Tetraspanin 18 (extracellular)
    • Product Code: ANR-189
    • Short Description: A Rabbit Polyclonal antibody to Tetraspanin 18 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: TSPAN18, Tetraspanin-18
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