Inflammation

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Anti-Robo2 (extracellular) Antibody
    Anti-Robo2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Robo2
    • Product Code: ANR-182
    • Short Description: A Rabbit Polyclonal Antibody to Robo2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot
    • Target: Roundabout homolog 2, Roundabout Guidance Receptor 2
  • Anti-Robo1 (extracellular)-FITC Antibody
    Anti-Robo1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Robo1 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ANR-181-F
    • Short Description: A Rabbit Polyclonal Antibody to Robo1 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Roundabout Guidance Receptor 1, Roundabout Homolog 1, Deleted In U Twenty Twenty, DUTT1
  • Anti-Robo1 (extracellular) Antibody
    Anti-Robo1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Robo1
    • Product Code: ANR-181
    • Short Description: A Rabbit Polyclonal Antibody to Robo1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Roundabout Guidance Receptor 1, Roundabout Homolog 1, Deleted In U Twenty Twenty, DUTT1
  • Anti-Progranulin Antibody
    Anti-Progranulin Antibody
    A Rabbit Polyclonal antibody to Progranulin
    • Product Code: ANR-178
    • Short Description: A Rabbit Polyclonal antibody to Progranulin
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Granulin Precursor, PGRN, Epithelin Precursor, Glycoprotein 88, GP88, PC Cell-Derived Growth Factor, PCDGF, GRN
  • Anti-TMEM106B (extracellular) Antibody
    Anti-TMEM106B (extracellular) Antibody
    A Rabbit Polyclonal antibody to TMEM106B (extracellular)
    • Product Code: ANR-177
    • Short Description: A Rabbit Polyclonal antibody to TMEM106B (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Transmembrane Protein 106B
  • Anti-RAGE (extracellular) Antibody
    Anti-RAGE (extracellular) Antibody
    A Rabbit Polyclonal Antibody to RAGE
    • Product Code: ANR-176
    • Short Description: A Rabbit Polyclonal Antibody to RAGE
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot
    • Target: Advanced Glycosylation End Product-Specific Receptor, AGER, Receptor For Advanced Glycosylation End Products, SCARJ1
  • Anti-TMEM119 (extracellular)-FITC Antibody
    Anti-TMEM119 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to TMEM119 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ANR-175-F
    • Short Description: A Rabbit Polyclonal Antibody to TMEM119 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Rat, Mouse
    • Applications: Flow cytometry
    • Target: Transmembrane Protein 119, Osteoblast induction factor, OBIF
  • Anti-TMEM119 (extracellular)-APC Antibody
    Anti-TMEM119 (extracellular)-APC Antibody
    A Rabbit Polyclonal Antibody to TMEM119 (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Product Code: ANR-175-APC
    • Short Description: A Rabbit Polyclonal Antibody to TMEM119 (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Host: Rabbit
    • Label: APC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Transmembrane Protein 119, Osteoblast induction factor, OBIF
  • Anti-TMEM119 (extracellular) Antibody
    Anti-TMEM119 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to TMEM119 (extracellular)
    • Product Code: ANR-175
    • Short Description: A Rabbit Polyclonal Antibody to TMEM119 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot
    • Target: Transmembrane Protein 119, Osteoblast induction factor, OBIF
  • Anti-Contactin-2/CNTN2 (extracellular) Antibody
    Anti-Contactin-2/CNTN2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Contactin-2 (extracellular)
    • Product Code: ANR-172
    • Short Description: A Rabbit Polyclonal Antibody to Contactin-2 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Axonal glycoprotein TAG-1, Axonin-1, Transient axonal glycoprotein 1, TAX-1
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