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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Anti-Neuropilin-2 (NRP2) (extracellular)-FITC Antibody
    Anti-Neuropilin-2 (NRP2) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Neuropilin-2 Conjugated to the Fluorescent Dye FITC
    • Product Code: ANR-062-F
    • Short Description: A Rabbit Polyclonal Antibody to Neuropilin-2 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: Vascular endothelial cell growth factor 165 receptor 2, VEGF165R2
  • Anti-Neuropilin-2 (NRP2) (extracellular) Antibody
    Anti-Neuropilin-2 (NRP2) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Neuropilin-2
    • Product Code: ANR-062
    • Short Description: A Rabbit Polyclonal Antibody to Neuropilin-2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Vascular endothelial cell growth factor 165 receptor 2, VEGF165R2
  • Anti-Nectin-2/PVRL2 (extracellular) Antibody
    Anti-Nectin-2/PVRL2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Nectin-2/PVRL2
    • Product Code: ANR-052
    • Short Description: A Rabbit Polyclonal Antibody to Nectin-2/PVRL2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Poliovirus receptor-related protein 2, Herpes virus entry mediator B, PRR2, PVRR2, CD112
  • Anti-Nectin-1/PVRL1 (extracellular)-FITC Antibody
    Anti-Nectin-1/PVRL1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to TRPM2 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ANR-051-F
    • Short Description: A Rabbit Polyclonal Antibody to TRPM2 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Poliovirus receptor-related protein 1, Herpes virus entry mediator C, PRR1, CD111
  • Anti-Nectin-1/PVRL1 (extracellular) Antibody
    Anti-Nectin-1/PVRL1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Nectin-1/PVRL1
    • Product Code: ANR-051
    • Short Description: A Rabbit Polyclonal Antibody to Nectin-1/PVRL1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Poliovirus receptor-related protein 1, Herpes virus entry mediator C, PRR1, CD111
  • Anti-L1CAM (extracellular) Antibody
    Anti-L1CAM (extracellular) Antibody
    A Rabbit Polyclonal antibody to L1CAM (extracellular)
    • Product Code: ANR-047
    • Short Description: A Rabbit Polyclonal antibody to L1CAM (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: L1 Cell Adhesion Molecule, Neural Cell Adhesion Molecule L1, NCAM-L1, CAML1, CD171
  • Anti-DSCAM (extracellular) Antibody
    Anti-DSCAM (extracellular) Antibody
    A Rabbit Polyclonal antibody to L1CAM (extracellular)
    • Product Code: ANR-046
    • Short Description: A Rabbit Polyclonal antibody to L1CAM (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunohistochemistry
    • Target: Down Syndrome Cell Adhesion Molecule, DS Cell Adhesion Molecule, CHD2
  • Anti-NrCAM (extracellular)-FITC Antibody
    Anti-NrCAM (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to NrCAM (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ANR-045-F
    • Short Description: A Rabbit Polyclonal Antibody to NrCAM (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Neuronal cell adhesion molecule, Nr-CAM, NgCAM-related cell adhesion molecule, Ankyrin-binding cell adhesion molecule, Neuronal surface protein Bravo, HBravo
  • Anti-NrCAM (extracellular) Antibody
    Anti-NrCAM (extracellular) Antibody
    A Rabbit Polyclonal Antibody to NrCAM
    • Product Code: ANR-045
    • Short Description: A Rabbit Polyclonal Antibody to NrCAM
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Neuronal cell adhesion molecule, Nr-CAM, NgCAM-related cell adhesion molecule, Ankyrin-binding cell adhesion molecule, Neuronal surface protein Bravo, HBravo
  • Anti-CD56/NCAM1 (extracellular)-ATTO Fluor-488 Antibody
    Anti-CD56/NCAM1 (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to CD56/NCAM1
    • Product Code: ANR-041-AG
    • Short Description: A Rabbit Polyclonal Antibody to CD56/NCAM1
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunohistochemistry
    • Target: NCAM-1, CD56
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