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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Guinea Pig Anti-Synaptotagmin-1 (SYT1) Antibody
    Guinea Pig Anti-Synaptotagmin-1 (SYT1) Antibody
    A Guinea Pig Polyclonal Antibody to Anti-Synaptotagmin-1 (SYT1)
    • Product Code: ANR-003-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Anti-Synaptotagmin-1 (SYT1)
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Synaptotagmin I, SytI, p65, SVP65
  • Anti-Synaptotagmin-1 (SYT1) Antibody
    Anti-Synaptotagmin-1 (SYT1) Antibody
    A Rabbit Polyclonal Antibody to Synaptotagmin-1
    • Product Code: ANR-003
    • Short Description: A Rabbit Polyclonal Antibody to Synaptotagmin-1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Synaptotagmin I, SytI, p65, SVP65
  • Anti-Syntaxin 1 Antibody
    Anti-Syntaxin 1 Antibody
    A Rabbit Polyclonal Antibody to Syntaxin 1
    • Product Code: ANR-002
    • Short Description: A Rabbit Polyclonal Antibody to Syntaxin 1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: STX1A, Synaptotagmin-associated 35 kDa protein, P35A, P35-1, Neuron-specific antigen HPC-1
  • Anti-SNAP-25 Antibody
    Anti-SNAP-25 Antibody
    A Rabbit Polyclonal Antibody to SNAP-25
    • Product Code: ANR-001
    • Short Description: A Rabbit Polyclonal Antibody to SNAP-25
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Synaptosomal-associated protein 25 kD
  • Anti-Alpha 3 Na<sup>+</sup>/K<sup>+</sup> ATPase Antibody
    Anti-Alpha 3 Na+/K+ ATPase Antibody
    A Rabbit Polyclonal Antibody to Alpha 3 Na+/K+ ATPase
    • Product Code: ANP-003
    • Short Description: A Rabbit Polyclonal Antibody to Alpha 3 Na+/K+ ATPase
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: ATP1A3, Sodium/potassium ATPase catalytic subunit alpha-3, Sodium pump subunit alpha-3, Na+/K+-transporting ATPase subunit alpha-3
  • Anti-Alpha 2 Na<sup>+</sup>/K<sup>+</sup> ATPase Antibody
    Anti-Alpha 2 Na+/K+ ATPase Antibody
    A Rabbit Polyclonal Antibody to Alpha 2 Na+/K+ ATPase
    • Product Code: ANP-002
    • Short Description: A Rabbit Polyclonal Antibody to Alpha 2 Na+/K+ ATPase
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: ATP1A2, Sodium/potassium ATPase catalytic subunit alpha-2, Sodium pump subunit alpha-2
  • Guinea Pig Anti-Alpha 1 Na<sup>+</sup>/K<sup>+</sup> ATPase Antibody
    Guinea Pig Anti-Alpha 1 Na+/K+ ATPase Antibody
    A Guinea Pig Polyclonal Antibody to Alpha 1 Na+/K+ ATPase
    • Product Code: ANP-001-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Alpha 1 Na+/K+ ATPase
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: ATP1A1, Sodium/potassium ATPase catalytic subunit alpha-1, Sodium pump subunit alpha-1
  • Anti-Alpha 1 Na<sup>+</sup>/K<sup>+</sup> ATPase Antibody
    Anti-Alpha 1 Na+/K+ ATPase Antibody
    A Rabbit Polyclonal Antibody to Alpha 1 Na+/K+ ATPase
    • Product Code: ANP-001
    • Short Description: A Rabbit Polyclonal Antibody to Alpha 1 Na+/K+ ATPase
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: ATP1A1, Sodium/potassium ATPase catalytic subunit alpha-1, Sodium pump subunit alpha-1
  • Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular)-ATTO Fluor-594 Antibody
    Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular)-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor β4 Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ANC-014-AR
    • Short Description: A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor β4 Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: nAChR β4, Neuronal acetylcholine receptor subunit beta-4, Cholinergic receptor nicotinic beta 4
  • Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular) Antibody
    Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor β4
    • Product Code: ANC-014
    • Short Description: A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor β4
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: nAChR β4, Neuronal acetylcholine receptor subunit beta-4, Cholinergic receptor nicotinic beta 4
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