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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2045 Results
  • Guinea Pig Anti-Alpha 1 Na<sup>+</sup>/K<sup>+</sup> ATPase Antibody
    Guinea Pig Anti-Alpha 1 Na+/K+ ATPase Antibody
    A Guinea Pig Polyclonal Antibody to Alpha 1 Na+/K+ ATPase
    • Product Code: ANP-001-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Alpha 1 Na+/K+ ATPase
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: ATP1A1, Sodium/potassium ATPase catalytic subunit alpha-1, Sodium pump subunit alpha-1
  • Anti-Alpha 1 Na<sup>+</sup>/K<sup>+</sup> ATPase Antibody
    Anti-Alpha 1 Na+/K+ ATPase Antibody
    A Rabbit Polyclonal Antibody to Alpha 1 Na+/K+ ATPase
    • Product Code: ANP-001
    • Short Description: A Rabbit Polyclonal Antibody to Alpha 1 Na+/K+ ATPase
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: ATP1A1, Sodium/potassium ATPase catalytic subunit alpha-1, Sodium pump subunit alpha-1
  • Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular)-ATTO Fluor-594 Antibody
    Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular)-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor β4 Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ANC-014-AR
    • Short Description: A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor β4 Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: nAChR β4, Neuronal acetylcholine receptor subunit beta-4, Cholinergic receptor nicotinic beta 4
  • Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular) Antibody
    Anti-Nicotinic Acetylcholine Receptor β4 (CHRNB4) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor β4
    • Product Code: ANC-014
    • Short Description: A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor β4
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: nAChR β4, Neuronal acetylcholine receptor subunit beta-4, Cholinergic receptor nicotinic beta 4
  • Anti-Nicotinic Acetylcholine Receptor β2 (CHRNB2) (extracellular)-ATTO Fluor-594 Antibody
    Anti-Nicotinic Acetylcholine Receptor β2 (CHRNB2) (extracellular)-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor β2 Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ANC-012-AR
    • Short Description: A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor β2 Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: nAChR β2, Neuronal acetylcholine receptor subunit beta-2, Cholinergic receptor nicotinic beta 2, ACRB2, EFNL3
  • Anti-Nicotinic Acetylcholine Receptor β2 (CHRNB2) (extracellular) Antibody
    Anti-Nicotinic Acetylcholine Receptor β2 (CHRNB2) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor β2
    • Product Code: ANC-012
    • Short Description: A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor β2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: nAChR β2, Neuronal acetylcholine receptor subunit beta-2, Cholinergic receptor nicotinic beta 2, ACRB2, EFNL3
  • Guinea Pig Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular) Antibody
    Guinea Pig Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular) Antibody
    A Guinea Pig Polyclonal Antibody to Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular)
    • Product Code: ANC-007-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular)
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: nAChR α7, Cholinergic receptor nicotinic alpha 7, Neuronal acetylcholine receptor subunit alpha-7, ACRA7
  • Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular)-ATTO Fluor-633 Antibody
    Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular)-ATTO Fluor-633 Antibody
    A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor α7 Conjugated to the Fluorescent Dye ATTO-633
    • Product Code: ANC-007-FR
    • Short Description: A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor α7 Conjugated to the Fluorescent Dye ATTO-633
    • Host: Rabbit
    • Label: ATTO 633
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: nAChR α7, Cholinergic receptor nicotinic alpha 7, Neuronal acetylcholine receptor subunit alpha-7, ACRA7
  • Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular)-FITC Antibody
    Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor α7 Conjugated to the Fluorescent Dye FITC
    • Product Code: ANC-007-F
    • Short Description: A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor α7 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: nAChR α7, Cholinergic receptor nicotinic alpha 7, Neuronal acetylcholine receptor subunit alpha-7, ACRA7
  • Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular) Antibody
    KO Validate'
    Anti-Nicotinic Acetylcholine Receptor α7 (CHRNA7) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor α7
    • Product Code: ANC-007
    • Short Description: A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor α7
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: nAChR α7, Cholinergic receptor nicotinic alpha 7, Neuronal acetylcholine receptor subunit alpha-7, ACRA7
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