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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2045 Results
  • Anti-Nicotinic Acetylcholine Receptor α5 (CHRNA5) (extracellular) Antibody
    Anti-Nicotinic Acetylcholine Receptor α5 (CHRNA5) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor α5
    • Product Code: ANC-005
    • Short Description: A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor α5
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: nAChR α5, Cholinergic receptor nicotinic alpha 5, NACHRA5, Neuronal acetylcholine receptor subunit alpha-5
  • Anti-Nicotinic Acetylcholine Receptor α4 (CHRNA4) (extracellular) Antibody
    KO Validate'
    Anti-Nicotinic Acetylcholine Receptor α4 (CHRNA4) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor α4
    • Product Code: ANC-004
    • Short Description: A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor α4
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: nAChR α4, Cholinergic receptor nicotinic alpha 4, NACRA4, NACHRA4, Neuronal acetylcholine receptor subunit alpha-4
  • Anti-Nicotinic Acetylcholine Receptor α2 (CHRNA2) (extracellular) Antibody
    Anti-Nicotinic Acetylcholine Receptor α2 (CHRNA2) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor α2
    • Product Code: ANC-002
    • Short Description: A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor α2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: nAChR α2, Cholinergic receptor nicotinic alpha 2, Neuronal acetylcholine receptor subunit alpha-2
  • Anti-NGF Antibody
    Anti-NGF Antibody
    A Rabbit Polyclonal Antibody to Nerve Growth Factor
    • Product Code: AN-240
    • Short Description: A Rabbit Polyclonal Antibody to Nerve Growth Factor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Nerve growth factor
  • Mouse Anti-Rat p75 NGF Receptor (extracellular)-FITC Antibody
    Mouse Anti-Rat p75 NGF Receptor (extracellular)-FITC Antibody
    A Mouse Monoclonal Antibody to Rat Neurotrophin Receptor p75NTR Conjugated to the Fluorescent Dye FITC
    • Product Code: AN-170-F
    • Short Description: A Mouse Monoclonal Antibody to Rat Neurotrophin Receptor p75NTR Conjugated to the Fluorescent Dye FITC
    • Host: Mouse
    • Label: FITC
    • Reactivity: Rat
    • Applications: Flow cytometry
    • Target: p75NTR, NGFR p75, TNFR superfamily member 16, TNFRSF16, CD271
  • Mouse Anti-Rat p75 NGF Receptor (extracellular) Antibody
    Mouse Anti-Rat p75 NGF Receptor (extracellular) Antibody
    A Mouse Monoclonal Antibody to Rat Neurotrophin Receptor p75NTR
    • Product Code: AN-170
    • Short Description: A Mouse Monoclonal Antibody to Rat Neurotrophin Receptor p75NTR
    • Host: Mouse
    • Label: Unconjugated
    • Reactivity: Rat
    • Applications: Indirect flow cytometry, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: p75NTR, NGFR p75, TNFR superfamily member 16, TNFRSF16, CD271
  • Anti-ABCB4/MDR3 Antibody
    Anti-ABCB4/MDR3 Antibody
    A Rabbit Polyclonal Antibody to ABCB4/MDR3
    • Product Code: AMT-023
    • Short Description: A Rabbit Polyclonal Antibody to ABCB4/MDR3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: ATP binding cassette subfamily B member 4, Multidrug resistance protein 3, P-glycoprotein 2, Pgy2
  • Anti-Human ABCB1/MDR1 Antibody
    Anti-Human ABCB1/MDR1 Antibody
    A Rabbit Polyclonal Antibody to Multidrug Resistance Protein 1
    • Product Code: AMT-021
    • Short Description: A Rabbit Polyclonal Antibody to Multidrug Resistance Protein 1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Western blot
    • Target: Multidrug resistance protein 1, ATP-binding cassette sub-family B (MDR/TAP) member 1, P-glycoprotein 1, P-gp, PGY1, ABC20, CD243, CLCS, Colchicin sensitivity, Doxorubicin resistance, GP170
  • Anti-MCT4 (SLC16A3) Antibody
    Anti-MCT4 (SLC16A3) Antibody
    A Rabbit Polyclonal Antibody to Monocarboxylate Transporter 4
    • Product Code: AMT-014
    • Short Description: A Rabbit Polyclonal Antibody to Monocarboxylate Transporter 4
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Monocarboxylate transporter 4, Monocarboxylic acid transporter 4, MOT4
  • Anti-MCT1 (SLC16A1) (extracellular)-FITC Antibody
    Anti-MCT1 (SLC16A1) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Monocarboxylate Transporter 1 Conjugated to the Fluorescent Dye FITC
    • Product Code: AMT-011-F
    • Short Description: A Rabbit Polyclonal Antibody to Monocarboxylate Transporter 1 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: Monocarboxylate transporter 1, Monocarboxylic acid transporter 1
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