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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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2041 Results
  • Anti-Melatonin Receptor 1A (MTNR1A) Antibody
    Anti-Melatonin Receptor 1A (MTNR1A) Antibody
    A Rabbit Polyclonal Antibody to Melatonin Receptor Type 1A
    • Product Code: AMR-031
    • Short Description: A Rabbit Polyclonal Antibody to Melatonin Receptor Type 1A
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: MT1, Mel1a receptor, Mel-1A-R
  • Anti-MC4 Receptor (extracellular) Antibody
    KO Validate'
    Anti-MC4 Receptor (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Melanocortin Receptor 4
    • Product Code: AMR-024
    • Short Description: A Rabbit Polyclonal Antibody to Melanocortin Receptor 4
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Melanocortin receptor 4, MC4R, MC4-R
  • Anti-MC3 Receptor (extracellular) Antibody
    Anti-MC3 Receptor (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Melanocortin Receptor 3
    • Product Code: AMR-023
    • Short Description: A Rabbit Polyclonal Antibody to Melanocortin Receptor 3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Melanocortin receptor 3, MC3R, MC3-R
  • Anti-MC2 Receptor (extracellular) Antibody
    Anti-MC2 Receptor (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Melanocortin Receptor 2
    • Product Code: AMR-022
    • Short Description: A Rabbit Polyclonal Antibody to Melanocortin Receptor 2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Melanocortin receptor 2, MC2R, MC2-R, Adrenocorticotropic hormone receptor, ACTH receptor, ACTHR, ACTH-R, Adrenocorticotropin receptor
  • Anti-MC1 Receptor Antibody
    KO Validate'
    Anti-MC1 Receptor Antibody
    A Rabbit Polyclonal Antibody to Melanocortin Receptor 1
    • Product Code: AMR-020
    • Short Description: A Rabbit Polyclonal Antibody to Melanocortin Receptor 1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Melanocortin receptor 1, MC1R, MC1-R, Melanocyte-stimulating hormone receptor, MSHR, MSH-R, SHEP2
  • Anti-CHRM3 (extracellular) Antibody
    Anti-CHRM3 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CHRM3 (extracellular)
    • Product Code: AMR-013
    • Short Description: A Rabbit Polyclonal Antibody to CHRM3 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunohistochemistry
    • Target: CHRM3
  • Anti-CHRM1 (443-458) Antibody
    Anti-CHRM1 (443-458) Antibody
    A Rabbit Polyclonal Antibody to Muscarinic Acetylcholine Receptor M1
    • Product Code: AMR-010
    • Short Description: A Rabbit Polyclonal Antibody to Muscarinic Acetylcholine Receptor M1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Cholinergic muscarinic receptor 1, Muscarinic acetylcholine receptor M1
  • Guinea Pig Anti-CHRM3 Antibody
    Guinea Pig Anti-CHRM3 Antibody
    A Guinea Pig Polyclonal Antibody to Acetylcholine receptor muscarinic 3
    • Product Code: AMR-006-GP
    • Short Description: A Guinea Pig Polyclonal Antibody to Acetylcholine receptor muscarinic 3
    • Host: Guinea Pig
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Cholinergic receptor muscarinic 3, Acetylcholine receptor muscarinic 3, M3 AChR
  • Anti-CHRM3 Antibody
    KO Validate'
    Anti-CHRM3 Antibody
    A Rabbit Polyclonal Antibody to Muscarinic Acetylcholine Receptor M3
    • Product Code: AMR-006
    • Short Description: A Rabbit Polyclonal Antibody to Muscarinic Acetylcholine Receptor M3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Cholinergic muscarinic receptor 3, Muscarinic acetylcholine receptor M3, M3 mAChR
  • Anti-CHRM2 Antibody
    KO Validate'
    Anti-CHRM2 Antibody
    A Rabbit Polyclonal Antibody to Muscarinic Acetylcholine Receptor M2
    • Product Code: AMR-002
    • Short Description: A Rabbit Polyclonal Antibody to Muscarinic Acetylcholine Receptor M2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Muscarinic acetylcholine receptor M2, Cholinergic receptor muscarinic 2, mAChR M2
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