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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Anti-CHRM1 Antibody
    KO Validate'
    Anti-CHRM1 Antibody
    A Rabbit Polyclonal Antibody to Muscarinic Acetylcholine Receptor M1
    • Product Code: AMR-001
    • Short Description: A Rabbit Polyclonal Antibody to Muscarinic Acetylcholine Receptor M1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Cholinergic muscarinic receptor 1, Muscarinic acetylcholine receptor M1
  • Anti-CLEC7A/Dectin-1 (extracellular)-FITC Antibody
    Anti-CLEC7A/Dectin-1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to CLEC7A (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ALR-062-F
    • Short Description: A Rabbit Polyclonal Antibody to CLEC7A (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: C-Type Lectin Domain Family 7 Member A, Dendritic Cell-Associated C-Type Lectin 1, DC-Associated C-Type Lectin 1, Beta-Glucan Receptor, C-Type Lectin Superfamily Member 12, CLECSF12, CD369, SCARE2
  • Anti-CLEC7A/Dectin-1 (extracellular) Antibody
    Anti-CLEC7A/Dectin-1 (extracellular) Antibody
    A Rabbit Polyclonal antibody to CLEC7A/Dectin-1 (extracellular)
    • Product Code: ALR-062
    • Short Description: A Rabbit Polyclonal antibody to CLEC7A/Dectin-1 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: C-Type Lectin Domain Family 7 Member A, Dendritic Cell-Associated C-Type Lectin 1, DC-Associated C-Type Lectin 1, Beta-Glucan Receptor, C-Type Lectin Superfamily Member 12, CLECSF12, CD369
  • Anti-LGI1 Antibody
    Anti-LGI1 Antibody
    A Rabbit Polyclonal Antibody to LGI1
    • Product Code: ALR-041
    • Short Description: A Rabbit Polyclonal Antibody to LGI1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: Leucine-rich glioma-inactivated protein 1, Epitempin-1, EPT
  • Anti-Leptin Receptor (extracellular)-FITC Antibody
    Anti-Leptin Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal antibody to Leptin Receptor (extracellular) conjugated to the fluorescent dye FITC.
    • Product Code: ALR-039-F
    • Short Description: A Rabbit Polyclonal antibody to Leptin Receptor (extracellular) conjugated to the fluorescent dye FITC.
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: LEPR, OB receptor, OBR, CD295
  • Anti-Leptin Receptor (extracellular) Antibody
    Anti-Leptin Receptor (extracellular) Antibody
    A Rabbit Polyclonal antibody to Leptin Receptor (extracellular)
    • Product Code: ALR-039
    • Short Description: A Rabbit Polyclonal antibody to Leptin Receptor (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: LEPR, OB receptor, OBR, CD295
  • Anti-LPAR6 (P2RY5) (extracellular) Antibody
    Anti-LPAR6 (P2RY5) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to LPA Receptor 6 (P2RY5)
    • Product Code: ALR-036
    • Short Description: A Rabbit Polyclonal Antibody to LPA Receptor 6 (P2RY5)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: Lysophosphatidic acid receptor 6, LPA receptor 6, LPA-6, P2Y purinoceptor 5, Purinergic receptor 5, P2Y5, RB intron encoded G-protein coupled receptor
  • Anti-Human LPAR5 (extracellular) Antibody
    Anti-Human LPAR5 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to LPA Receptor 5
    • Product Code: ALR-035
    • Short Description: A Rabbit Polyclonal Antibody to LPA Receptor 5
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Western blot
    • Target: Lysophosphatidic acid receptor 5, LPA-5, GPR92, GPR93
  • Anti-LPAR4 (P2Y9) (extracellular) Antibody
    Anti-LPAR4 (P2Y9) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to LPA Receptor 4 (P2Y9)
    • Product Code: ALR-034
    • Short Description: A Rabbit Polyclonal Antibody to LPA Receptor 4 (P2Y9)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Lysophosphatidic acid receptor 4, LPA receptor 4, LPA-4, P2Y purinoceptor 9, Purinergic receptor 9, P2RY9, GPR23
  • Anti-LPAR3 (EDG7) (extracellular) Antibody
    Anti-LPAR3 (EDG7) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to LPA Receptor 3 (EDG7)
    • Product Code: ALR-033
    • Short Description: A Rabbit Polyclonal Antibody to LPA Receptor 3 (EDG7)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Lysophosphatidic acid receptor 3, LPA receptor 3, LPA-3, snGPCR32
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