Inflammation

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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2044 Results
  • Recombinant human LIF protein
    Bioassay Tested
    Recombinant human LIF protein
    A recombinant human Leukemia Inhibitory Factor (LIF) cytokine that promotes stem cell self-renewal and inhibits differentiation by signaling through the LIFR/gp130 receptor complex
    • Product Code: L-200
    • Short Description: A recombinant human Leukemia Inhibitory Factor (LIF) cytokine that promotes stem cell self-renewal and inhibits differentiation by signaling through the LIFR/gp130 receptor complex
    • Applications: Cell Culture, Proliferation assay, Western blot
    • MW: 19.7 kDa
  • Recombinant human GDNF protein
    Bioassay Tested
    Recombinant human GDNF protein
    A recombinant human glial cell line-derived neurotrophic factor (GDNF) that enhances the survival and differentiation of dopaminergic and motor neurons via GFRα1/RET receptor complex
    • Product Code: G-240
    • Short Description: A recombinant human glial cell line-derived neurotrophic factor (GDNF) that enhances the survival and differentiation of dopaminergic and motor neurons via GFRα1/RET receptor complex
    • Applications: Axon growth, Cell Culture, Western blot
    • MW: 30.1 kDa (dimer)
  • Recombinant human FGF-b protein
    Bioassay Tested
    Recombinant human FGF-b protein
    A recombinant human growth factor (FGF-2/bFGF) that promotes cell proliferation, angiogenesis, and neuronal survival via FGFR receptors
    • Product Code: F-170
    • Short Description: A recombinant human growth factor (FGF-2/bFGF) that promotes cell proliferation, angiogenesis, and neuronal survival via FGFR receptors
    • Applications: Cell Culture, Neurosphere formation, Proliferation assay
    • MW: 16.5 kDa
  • Recombinant human EGF protein
    Bioassay Tested
    Recombinant human EGF protein
    A recombinant human EGF that stimulates cell proliferation and survival via EGFR binding
    • Product Code: E-100
    • Short Description: A recombinant human EGF that stimulates cell proliferation and survival via EGFR binding
    • Applications: Cell Culture, Western blot
    • MW: 6.2 kDa
  • Na<sub>V</sub>1.7-Compound 36
    Bioassay Tested
    NaV1.7-Compound 36
    A Potent and Selective Blocker of NaV1.7 Channels
    • Product Code: CMN-003
    • Short Description: A Potent and Selective Blocker of NaV1.7 Channels
    • CAS No.: 1694633-51-6
    • MW: 486.85
  • Recombinant rat CNTF protein
    Bioassay Tested
    Recombinant rat CNTF protein
    A potent survival factor for neurons and oligodendrocytes
    • Product Code: C-245
    • Short Description: A potent survival factor for neurons and oligodendrocytes
    • Applications: Cell Culture, Western blot
    • MW: 22.7 kDa
  • Recombinant human CNTF protein
    Bioassay Tested
    Recombinant human CNTF protein
    A potent survival factor for neurons and oligodendrocytes
    • Product Code: C-240
    • Short Description: A potent survival factor for neurons and oligodendrocytes
    • Applications: Cell Culture, Western blot
    • MW: 22.9 kDa
  • Recombinant human Cardiotrophin-1 protein
    Bioassay Tested
    Recombinant human Cardiotrophin-1 protein
    A recombinant human cytokine that promotes cardiac myocyte hypertrophy and survival, and acts as a neurotrophic factor via the gp130/LIFR receptor complex
    • Product Code: C-200
    • Short Description: A recombinant human cytokine that promotes cardiac myocyte hypertrophy and survival, and acts as a neurotrophic factor via the gp130/LIFR receptor complex
    • Applications: Cell Culture, Western blot
    • MW: 21.1 kDa
  • Recombinant human Val66Met proBDNF protein
    Bioassay Tested
    Recombinant human Val66Met proBDNF protein
    A human proBDNF variant (Met66) for the study of p75NTR and sortilin receptor signaling
    • Product Code: B-457
    • Short Description: A human proBDNF variant (Met66) for the study of p75NTR and sortilin receptor signaling
    • Applications: Cell Culture, Western blot
    • MW: 51.45 kDa (dimer)
  • Recombinant human Val66Met proBDNF (cleavage resistant) protein
    Bioassay Tested
    Recombinant human Val66Met proBDNF (cleavage resistant) protein
    A cleavage-resistant human proBDNF variant (Met66) for the study of p75NTR and sortilin receptor signaling
    • Product Code: B-456
    • Short Description: A cleavage-resistant human proBDNF variant (Met66) for the study of p75NTR and sortilin receptor signaling
    • Applications: Cell Culture, Western blot
    • MW: 51.45 kDa (dimer)
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