Inflammation

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Anti-P2Y12 Receptor (extracellular)-FITC Antibody
    Anti-P2Y12 Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to P2Y12 Receptor Conjugated to the Fuorescent dye FITC
    • Product Code: APR-020-F
    • Short Description: A Rabbit Polyclonal Antibody to P2Y12 Receptor Conjugated to the Fuorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: P2Y purinoceptor 12, P2RY12, ADP-glucose receptor, Platelet ADP receptor, HORK3
  • Anti-P2X7 Receptor (extracellular)-FITC Antibody
    KO Validate'
    Anti-P2X7 Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to P2X7 Receptor Conjugated to the Fluorescent Dye FITC
    • Product Code: APR-008-F
    • Short Description: A Rabbit Polyclonal Antibody to P2X7 Receptor Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Immunocytochemistry, Immunofluorescence, Flow cytometry
    • Target: Purinergic receptor P2X7, P2RX7, P2Z, ATP receptor
  • Anti-P2X7 Receptor (extracellular) Antibody
    KO Validate'
    Anti-P2X7 Receptor (extracellular) Antibody
    A Rabbit Polyclonal Antibody to P2X7 Receptor
    • Product Code: APR-008
    • Short Description: A Rabbit Polyclonal Antibody to P2X7 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Purinergic receptor P2X7, P2RX7, P2Z, ATP receptor
  • Anti-K<sub>V</sub>1.2 (KCNA2) Antibody
    Anti-KV1.2 (KCNA2) Antibody
    A Rabbit Polyclonal Antibody to KV1.2 (KCNA2) Channel
    • Product Code: APC-010
    • Short Description: A Rabbit Polyclonal Antibody to KV1.2 (KCNA2) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Potassium voltage-gated channel subfamily A member 2, RBK2
  • Anti-TrkB (extracellular) Antibody
    Anti-TrkB (extracellular) Antibody
    A Rabbit Polyclonal Antibody to TrkB
    • Product Code: ANT-019
    • Short Description: A Rabbit Polyclonal Antibody to TrkB
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Neurotrophic tyrosine kinase receptor type 2, NTRK2, BDNF/NT-3 growth factors receptor
  • Anti-TrkA (extracellular) Antibody
    KO Validate'
    Anti-TrkA (extracellular) Antibody
    A Rabbit Polyclonal Antibody to TrkA
    • Product Code: ANT-018
    • Short Description: A Rabbit Polyclonal Antibody to TrkA
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: High affinity nerve growth factor receptor, Neurotrophic tyrosine kinase receptor type 1, NTRK1
  • Anti-p75 NGF Receptor (extracellular) Antibody
    KO Validate'
    Anti-p75 NGF Receptor (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Human Neurotrophin Receptor p75NTR
    • Product Code: ANT-007
    • Short Description: A Rabbit Polyclonal Antibody to Human Neurotrophin Receptor p75NTR
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: p75NTR, p75 Neurotrophin receptor, Low affinity NGF receptor, TNFR superfamily member 16, TNFRSF16, CD271
  • Anti-Nicotinic Acetylcholine Receptor α3 (CHRNA3) (extracellular) Antibody
    Anti-Nicotinic Acetylcholine Receptor α3 (CHRNA3) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor α3
    • Product Code: ANC-003
    • Short Description: A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor α3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: nAChR α3, Neuronal acetylcholine receptor subunit alpha-3, ACHA3, PAOD2
  • Anti-GABA(A) α1 Receptor (extracellular)-FITC Antibody
    Anti-GABA(A) α1 Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to GABRA1 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: AGA-001-F
    • Short Description: A Rabbit Polyclonal Antibody to GABRA1 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: γ-Aminobutyric acid receptor type A subunit α1, GABRA1
  • α-Dendrotoxin-ATTO Fluor-647N
    Bioassay Tested
    α-Dendrotoxin-ATTO Fluor-647N
    A fluorescent probe for imaging and sensitive detection of Kv1.1, Kv1.2, and Kv1.6 channels.
    • Product Code: D-350-FRN
    • Short Description: A fluorescent probe for imaging and sensitive detection of Kv1.1, Kv1.2, and Kv1.6 channels.
    • Label: ATTO 647N
    • Applications: Electrophysiology, Direct flow cytometry, Fluorescence staining, Live cell imaging, Immunofluorescence
    • MW: ~7990 Da
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