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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Slotoxin
    Bioassay Tested
    Slotoxin
    A potent and selective blocker of KCa1.1 (BK) channels
    • Product Code: STS-410
    • Short Description: A potent and selective blocker of KCa1.1 (BK) channels
    • Applications: Electrophysiology
    • CAS No.: 401470-29-9
    • MW: 4085.9 Da
  • ShK (Stichodactyla Toxin)
    Bioassay Tested
    ShK (Stichodactyla Toxin)
    A potent blocker of multiple KV1 channels (KV1.1, KV1.3, KV1.4, KV1.6) and KCa3.1 channels.
    • Product Code: STS-400
    • Short Description: A potent blocker of multiple KV1 channels (KV1.1, KV1.3, KV1.4, KV1.6) and KCa3.1 channels.
    • Applications: Electrophysiology
    • CAS No.: 172450-46-3
    • MW: 4054.8 Da
  • ShK-186
    Bioassay Tested
    ShK-186
    A potent and highly selective blocker of the KV1.3 K+ channels (a T-cell ion-channel blocker)
    • Product Code: STS-390
    • Short Description: A potent and highly selective blocker of the KV1.3 K+ channels (a T-cell ion-channel blocker)
    • Applications: Electrophysiology
    • CAS No.: 1081110-69-1
    • MW: 4441 Da
  • Scyllatoxin
    Bioassay Tested
    Scyllatoxin
    A potent and selective blocker of KCa2.2 and KCa2.3 (SK) K+ channels
    • Product Code: STS-370
    • Short Description: A potent and selective blocker of KCa2.2 and KCa2.3 (SK) K+ channels
    • Applications: Electrophysiology
    • CAS No.: 142948-19-4
    • MW: 3423 Da
  • Stromatoxin-1
    Bioassay Tested
    Stromatoxin-1
    A potent blocker of KV2.1, KV2.2, KV4.2 and KV9.3 channels
    • Product Code: STS-350
    • Short Description: A potent blocker of KV2.1, KV2.2, KV4.2 and KV9.3 channels
    • Applications: Electrophysiology
    • CAS No.: 741738-59-0
    • MW: 3791.4 Da
  • Phlotoxin 1
    Bioassay Tested
    Phlotoxin 1
    A potent blocker of NaV1.7 channels and inhibitor of other NaV subtypes
    • Product Code: STP-800
    • Short Description: A potent blocker of NaV1.7 channels and inhibitor of other NaV subtypes
    • Applications: Electrophysiology
    • MW: 4057.7 Da
  • Phrixotoxin-3
    Bioassay Tested
    Phrixotoxin-3
    A potent blocker of NaV1.2 and NaV1.6 channels
    • Product Code: STP-720
    • Short Description: A potent blocker of NaV1.2 and NaV1.6 channels
    • Applications: Electrophysiology
    • CAS No.: 880886-00-0
    • MW: 4058.8 Da
  • Phrixotoxin-2
    Bioassay Tested
    Phrixotoxin-2
    A potent blocker/gating modifier of KV4.2 and KV4.3 channels
    • Product Code: STP-710
    • Short Description: A potent blocker/gating modifier of KV4.2 and KV4.3 channels
    • Applications: Electrophysiology
    • CAS No.: 221889-63-0
    • MW: 3921.7 Da
  • Phrixotoxin-1
    Bioassay Tested
    Phrixotoxin-1
    A potent blocker/gating modifier of KV4.2 and KV4.3 channels
    • Product Code: STP-700
    • Short Description: A potent blocker/gating modifier of KV4.2 and KV4.3 channels
    • Applications: Electrophysiology
    • CAS No.: 221872-97-5
    • MW: 3548.4 Da
  • Pandinotoxin Kα
    Bioassay Tested
    Pandinotoxin Kα
    A blocker of KV1.2, KV3, KV1.4 and KV2 K+ channels
    • Product Code: STP-500
    • Short Description: A blocker of KV1.2, KV3, KV1.4 and KV2 K+ channels
    • Applications: Electrophysiology
    • CAS No.: 185529-64-0
    • MW: 4033 Da
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