Inflammation

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • Hm3a Toxin
    Bioassay Tested
    Hm3a Toxin
    A highly potent ASIC1a blocker and ASIC1b activator
    • Product Code: STH-250
    • Short Description: A highly potent ASIC1a blocker and ASIC1b activator
    • Applications: Electrophysiology
    • MW: 4287 Da
  • Hd1a Toxin
    Bioassay Tested
    Hd1a Toxin
    A potent and selective blocker of NaV1.7 channels
    • Product Code: STH-200
    • Short Description: A potent and selective blocker of NaV1.7 channels
    • Applications: Electrophysiology
    • MW: 3822.4 Da
  • Huwentoxin-XVI
    Bioassay Tested
    Huwentoxin-XVI
    A potent and selective blocker of neuronal N-type (CaV2.2) calcium channels
    • Product Code: STH-105
    • Short Description: A potent and selective blocker of neuronal N-type (CaV2.2) calcium channels
    • Applications: Electrophysiology
    • CAS No.: 1600543-88-1
    • MW: 4437 Da
  • m3-Huwentoxin IV
    Bioassay Tested
    m3-Huwentoxin IV
    A highly potent blocker of the neuronal NaV1.7 voltage-gated sodium channels, with high affinity for other neuronal TTX-sensitive (NaV) subtypes
    • Product Code: STH-102
    • Short Description: A highly potent blocker of the neuronal NaV1.7 voltage-gated sodium channels, with high affinity for other neuronal TTX-sensitive (NaV) subtypes
    • Applications: Electrophysiology
    • MW: 3986 Da
  • Huwentoxin-I
    Bioassay Tested
    Huwentoxin-I
    A presynaptic blocker of N-type (CaV2.2) calcium channels, TTX-sensitive (NaV) sodium, and muscle nAChR channels
    • Product Code: STH-050
    • Short Description: A presynaptic blocker of N-type (CaV2.2) calcium channels, TTX-sensitive (NaV) sodium, and muscle nAChR channels
    • Applications: Electrophysiology
    • MW: 3750 Da
  • ω-Grammotoxin SIA
    Bioassay Tested
    ω-Grammotoxin SIA
    A potent blocker of N-type (CaV2.2) and P/Q-type (CaV2.1) voltage-gated calcium channels
    • Product Code: STG-450
    • Short Description: A potent blocker of N-type (CaV2.2) and P/Q-type (CaV2.1) voltage-gated calcium channels
    • Applications: Electrophysiology
    • CAS No.: 152617-90-8
    • MW: 4109.8 Da
  • GpTx-1
    Bioassay Tested
    GpTx-1
    A potent and selective blocker of the neuronal NaV1.7 voltage-gated sodium channels
    • Product Code: STG-400
    • Short Description: A potent and selective blocker of the neuronal NaV1.7 voltage-gated sodium channels
    • Applications: Electrophysiology
    • MW: 4073.9 Da
  • GsAF-II
    Bioassay Tested
    GsAF-II
    A potent gating modifier of tetrodotoxin-sensitive (TTX-s) neuronal voltage-gated sodium (NaV) channels that also blocks hERG1 potassium channels
    • Product Code: STG-350
    • Short Description: A potent gating modifier of tetrodotoxin-sensitive (TTX-s) neuronal voltage-gated sodium (NaV) channels that also blocks hERG1 potassium channels
    • Applications: Electrophysiology
    • MW: 3979.8 Da
  • GsAF-I
    Bioassay Tested
    GsAF-I
    A potent gating modifier of tetrodotoxin-sensitive (TTX-s) neuronal voltage-gated sodium (NaV) channels that also blocks hERG1 potassium channels
    • Product Code: STG-300
    • Short Description: A potent gating modifier of tetrodotoxin-sensitive (TTX-s) neuronal voltage-gated sodium (NaV) channels that also blocks hERG1 potassium channels
    • Applications: Electrophysiology
    • MW: 3707.5 Da
  • GrTx1
    Bioassay Tested
    GrTx1
    A potent gating modifier of tetrodotoxin-sensitive (TTX-s) neuronal voltage-gated sodium (NaV) channels that also blocks hERG1 potassium channels
    • Product Code: STG-250
    • Short Description: A potent gating modifier of tetrodotoxin-sensitive (TTX-s) neuronal voltage-gated sodium (NaV) channels that also blocks hERG1 potassium channels
    • Applications: Electrophysiology
    • MW: 3697.5 Da
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