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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2045 Results
  • Cono RPRF-amide
    Bioassay Tested
    Cono RPRF-amide
    A specific potentiator of ASIC3 channels
    • Product Code: STC-170
    • Short Description: A specific potentiator of ASIC3 channels
    • Applications: Electrophysiology
    • MW: 573.7 Da
  • ω-Conotoxin SVIB
    Bioassay Tested
    ω-Conotoxin SVIB
    A potent, reversible N-type (CaV2.2) and irreversible P/Q-type (CaV2.1) voltage-gated calcium channel blocker
    • Product Code: STC-160
    • Short Description: A potent, reversible N-type (CaV2.2) and irreversible P/Q-type (CaV2.1) voltage-gated calcium channel blocker
    • Applications: Electrophysiology
    • MW: 2739 Da
  • ω-Conotoxin MoVIA
    Bioassay Tested
    ω-Conotoxin MoVIA
    A potent and selective blocker of neuronal N-type (CaV2.2) calcium channels
    • Product Code: STC-150
    • Short Description: A potent and selective blocker of neuronal N-type (CaV2.2) calcium channels
    • Applications: Electrophysiology
    • MW: 3607 Da
  • α-Conotoxin TxID
    Bioassay Tested
    α-Conotoxin TxID
    A potent antagonist of α3β4 nAChRs that also inhibits α6/α3β4 nicotinic acetylcholine receptors
    • Product Code: STC-110
    • Short Description: A potent antagonist of α3β4 nAChRs that also inhibits α6/α3β4 nicotinic acetylcholine receptors
    • Applications: Electrophysiology
    • CAS No.: 1496617-64-1
    • MW: 1489.8 Da
  • Ceratotoxin-2
    Bioassay Tested
    Ceratotoxin-2
    A potent blocker of central nervous system voltage-gated Na+ channels
    • Product Code: STC-100
    • Short Description: A potent blocker of central nervous system voltage-gated Na+ channels
    • Applications: Electrophysiology
    • MW: 4092.7 Da
  • α-Conotoxin GIC
    Bioassay Tested
    α-Conotoxin GIC
    A potent and selective antagonist of α3β2 and α6-containing nicotinic acetylcholine receptors (nAChRs)
    • Product Code: STC-070
    • Short Description: A potent and selective antagonist of α3β2 and α6-containing nicotinic acetylcholine receptors (nAChRs)
    • Applications: Electrophysiology
    • MW: 1609.8 Da
  • Cn2 Toxin
    Bioassay Tested
    Cn2 Toxin
    A potent and selective activator of NaV1.6 channels
    • Product Code: STC-060
    • Short Description: A potent and selective activator of NaV1.6 channels
    • Applications: Electrophysiology
    • MW: 7588.6 Da
  • α-Conotoxin MII
    Bioassay Tested
    α-Conotoxin MII
    A potent and selective antagonist of neuronal α3β2 and α6β2*-containing nicotinic acetylcholine receptors (nAChRs)
    • Product Code: STC-040
    • Short Description: A potent and selective antagonist of neuronal α3β2 and α6β2*-containing nicotinic acetylcholine receptors (nAChRs)
    • Applications: Electrophysiology
    • CAS No.: 175735-93-0
    • MW: 1711 Da
  • α-Conotoxin BuIA
    Bioassay Tested
    α-Conotoxin BuIA
    A broad-spectrum nAChR antagonist used to kinetically distinguish between β2- and β4-containing receptors
    • Product Code: STC-030
    • Short Description: A broad-spectrum nAChR antagonist used to kinetically distinguish between β2- and β4-containing receptors
    • Applications: Electrophysiology
    • MW: 1311.6 Da
  • α-Conotoxin Bt1.8
    Bioassay Tested
    α-Conotoxin Bt1.8
    A potent and selective antagonist of neuronal α6/α3β2β3 and α3β2 nicotinic acetylcholine receptors (nAChRs)
    • Product Code: STC-020
    • Short Description: A potent and selective antagonist of neuronal α6/α3β2β3 and α3β2 nicotinic acetylcholine receptors (nAChRs)
    • Applications: Electrophysiology
    • MW: 1646 Da
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