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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

2041 Results
  • ATX-II
    Bioassay Tested
    ATX-II
    A potent activator of voltage-gated sodium NaV channels that primarily targets cardiac NaV1.5 channels by delaying their fast inactivation
    • Product Code: STA-700
    • Short Description: A potent activator of voltage-gated sodium NaV channels that primarily targets cardiac NaV1.5 channels by delaying their fast inactivation
    • Applications: Electrophysiology
    • CAS No.: 60748-45-0
    • MW: 4935 Da
  • ω-Agatoxin TK
    Bioassay Tested
    ω-Agatoxin TK
    A potent and selective blocker of P/Q-type (CaV2.1) calcium channels
    • Product Code: STA-530
    • Short Description: A potent and selective blocker of P/Q-type (CaV2.1) calcium channels
    • Applications: Electrophysiology
    • CAS No.: 158484-42-5
    • MW: 5273 Da
  • ω-Agatoxin IVA
    Bioassay Tested
    ω-Agatoxin IVA
    A potent and selective blocker of P/Q-type (CaV2.1) calcium channels
    • Product Code: STA-500
    • Short Description: A potent and selective blocker of P/Q-type (CaV2.1) calcium channels
    • Applications: Electrophysiology
    • CAS No.: 145017-83-0
    • MW: 5202 Da
  • Agitoxin-2
    Bioassay Tested
    Agitoxin-2
    A potent blocker of KV1.3, KV1.1, and KV1.6 voltage-gated potassium channels
    • Product Code: STA-420
    • Short Description: A potent blocker of KV1.3, KV1.1, and KV1.6 voltage-gated potassium channels
    • Applications: Electrophysiology
    • CAS No.: 168147-41-9
    • MW: 4091 Da
  • Anthopleurin-C
    Bioassay Tested
    Anthopleurin-C
    A potent cardiotoxin that delays the inactivation of NaV channels
    • Product Code: STA-400
    • Short Description: A potent cardiotoxin that delays the inactivation of NaV channels
    • Applications: Electrophysiology
    • MW: 4877.6 Da
  • Agitoxin-3
    Bioassay Tested
    Agitoxin-3
    A potent blocker of KV1.3, KV1.1, and KV1.6 voltage-gated potassium channels
    • Product Code: STA-390
    • Short Description: A potent blocker of KV1.3, KV1.1, and KV1.6 voltage-gated potassium channels
    • Applications: Electrophysiology
    • CAS No.: 155646-23-4
    • MW: 4101 Da
  • AmmTx3 Toxin
    Bioassay Tested
    AmmTx3 Toxin
    A specific blocker of KV channels (subtypes KV4.2 and KV4.3) that selectively targets native neuronal A-type K+ currents
    • Product Code: STA-305
    • Short Description: A specific blocker of KV channels (subtypes KV4.2 and KV4.3) that selectively targets native neuronal A-type K+ currents
    • Applications: Electrophysiology
    • MW: 3822 Da
  • Apamin
    Bioassay Tested
    Apamin
    A blocker of small conductance Ca2+-Activated K+ Channels (SK Type, KCa2)
    • Product Code: STA-200
    • Short Description: A blocker of small conductance Ca2+-Activated K+ Channels (SK Type, KCa2)
    • Applications: Electrophysiology
    • CAS No.: 24345-16-2
    • MW: 2027.3 Da
  • APETx2
    Bioassay Tested
    APETx2
    A potent blocker of ASIC3 and moderate blocker of NaV1.8 channels
    • Product Code: STA-160
    • Short Description: A potent blocker of ASIC3 and moderate blocker of NaV1.8 channels
    • Applications: Electrophysiology
    • CAS No.: 713544-47-9
    • MW: 4561 Da
  • AaH1 Toxin
    Bioassay Tested
    AaH1 Toxin
    A potent modulator of NaV channels that delays/slows fast inactivation
    • Product Code: STA-155
    • Short Description: A potent modulator of NaV channels that delays/slows fast inactivation
    • Applications: Electrophysiology
    • MW: 6804 Da
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