Neuroimmunology

Neuroimmunology

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

223 Results
  • GPR83 (extracellular) Blocking Peptide
    GPR83 (extracellular) Blocking Peptide
    A Blocking Peptide for Anti-GPR83 (extracellular) Antibody
    • Product Code: BLP-GR053
    • Short Description: A Blocking Peptide for Anti-GPR83 (extracellular) Antibody
    • Applications: Western blot, Immunohistochemistry
    • Target: GPR83
  • GPR84 (extracellular) Blocking Peptide
    GPR84 (extracellular) Blocking Peptide
    A Blocking Peptide for Anti-GPR84 (extracellular) Antibody
    • Product Code: BLP-GR052
    • Short Description: A Blocking Peptide for Anti-GPR84 (extracellular) Antibody
    • Applications: Western blot, Immunohistochemistry
    • Target: GPR84
  • GsAF-I
    Bioassay Tested
    GsAF-I
    A potent gating modifier of tetrodotoxin-sensitive (TTX-s) neuronal voltage-gated sodium (NaV) channels that also blocks hERG1 potassium channels
    • Product Code: STG-300
    • Short Description: A potent gating modifier of tetrodotoxin-sensitive (TTX-s) neuronal voltage-gated sodium (NaV) channels that also blocks hERG1 potassium channels
    • Applications: Electrophysiology
    • MW: 3707.5 Da
  • GsAF-II
    Bioassay Tested
    GsAF-II
    A potent gating modifier of tetrodotoxin-sensitive (TTX-s) neuronal voltage-gated sodium (NaV) channels that also blocks hERG1 potassium channels
    • Product Code: STG-350
    • Short Description: A potent gating modifier of tetrodotoxin-sensitive (TTX-s) neuronal voltage-gated sodium (NaV) channels that also blocks hERG1 potassium channels
    • Applications: Electrophysiology
    • MW: 3979.8 Da
  • GW-2580
    Bioassay Tested
    GW-2580
    An Inhibitor of Trk Receptors and cFMS/CSF-1 Receptor Kinases
    • Product Code: G-255
    • Short Description: An Inhibitor of Trk Receptors and cFMS/CSF-1 Receptor Kinases
    • CAS No.: 870483-87-7
    • MW: 366.41
  • GYKI 47409 (BDZ-g)
    Bioassay Tested
    GYKI 47409 (BDZ-g)
    An Antagonist of AMPA Receptors
    • Product Code: G-245
    • Short Description: An Antagonist of AMPA Receptors
    • CAS No.: 732278-52-3
    • MW: 407.49
  • Hainantoxin-III
    Bioassay Tested
    Hainantoxin-III
    A potent blocker of neuronal NaV 1.7 and NaV1.2 voltage-gated sodium channels, with activity on NaV1.3 and NaV1.1 subtypes
    • Product Code: STH-120
    • Short Description: A potent blocker of neuronal NaV 1.7 and NaV1.2 voltage-gated sodium channels, with activity on NaV1.3 and NaV1.1 subtypes
    • Applications: Electrophysiology
    • MW: 3608 Da
  • Hainantoxin-IV
    Bioassay Tested
    Hainantoxin-IV
    A potent, reversible blocker of neuronal NaV1.7 and other TTX-sensitive voltage-gated sodium channels
    • Product Code: STH-130
    • Short Description: A potent, reversible blocker of neuronal NaV1.7 and other TTX-sensitive voltage-gated sodium channels
    • Applications: Electrophysiology
    • MW: 3987.6 Da
  • HMA
    Bioassay Tested
    HMA
    An Antagonist of Na+/H+ Exchangers, P2X7 receptors and TRPA1, ASIC3 Channels
    • Product Code: H-130
    • Short Description: An Antagonist of Na+/H+ Exchangers, P2X7 receptors and TRPA1, ASIC3 Channels
    • CAS No.: 1428-95-1
    • MW: 311.77
  • Human GPR132 (extracellular) Blocking Peptide
    Human GPR132 (extracellular) Blocking Peptide
    A Blocking Peptide for Anti-Human GPR132 (extracellular) Antibody
    • Product Code: BLP-GR046
    • Short Description: A Blocking Peptide for Anti-Human GPR132 (extracellular) Antibody
    • Applications: Western blot, Immunohistochemistry
    • Target: GPR132
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