Neuroimmunology

Neuroimmunology

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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226 Results
  • Anti-GPR84 (extracellular)-FITC Antibody
    Anti-GPR84 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to GPR84 Conjugated to the Fluorescent Dye FITC
    • Product Code: AGR-052-F
    • Short Description: A Rabbit Polyclonal Antibody to GPR84 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: G-protein coupled receptor 84, Inflammation-related G-protein coupled receptor EX33
  • Anti-GPR84 (extracellular)-PE Antibody
    Anti-GPR84 (extracellular)-PE Antibody
    A Rabbit Polyclonal Antibody to GPR84 (extracellular) conjugated to the fluorescent dye Phycoerythrin (PE)
    • Product Code: AGR-052-PE
    • Short Description: A Rabbit Polyclonal Antibody to GPR84 (extracellular) conjugated to the fluorescent dye Phycoerythrin (PE)
    • Host: Rabbit
    • Label: R-PE
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: G-protein coupled receptor 84, Inflammation-related G-protein coupled receptor EX33
  • Anti-Human GPR132/G2A (extracellular) Antibody
    Anti-Human GPR132/G2A (extracellular) Antibody
    A Rabbit Polyclonal Antibody to GPR132
    • Product Code: AGR-046
    • Short Description: A Rabbit Polyclonal Antibody to GPR132
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: G protein-coupled receptor 132, G2 accumulation protein, G2A
  • Anti-Human GPR132/G2A (extracellular)-FITC Antibody
    Anti-Human GPR132/G2A (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Human GPR132 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: AGR-046-F
    • Short Description: A Rabbit Polyclonal Antibody to Human GPR132 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human
    • Applications: Live cell imaging, Flow cytometry
    • Target: G protein-coupled receptor 132, G2 accumulation protein, G2A
  • Anti-Kidins220 Antibody
    Anti-Kidins220 Antibody
    A Rabbit Polyclonal Antibody to Kidins220
    • Product Code: APZ-017
    • Short Description: A Rabbit Polyclonal Antibody to Kidins220
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Kinase D-interacting substrate of 220 kDa, Ankyrin repeat-rich membrane-spanning protein, ARMS
  • Anti-LRRC26 Antibody
    Anti-LRRC26 Antibody
    A Rabbit Polyclonal Antibody to LRRC26
    • Product Code: APC-070
    • Short Description: A Rabbit Polyclonal Antibody to LRRC26
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: Leucine-rich repeat-containing protein 26, BK channel auxiliary gamma subunit LRRC26, Cytokeratin-associated protein in cancer, CAPC
  • Anti-LRRC33 (extracellular) Antibody
    Anti-LRRC33 (extracellular) Antibody
    A Rabbit Polyclonal antibody to LRRC33 (extracellular)
    • Product Code: ALR-043
    • Short Description: A Rabbit Polyclonal antibody to LRRC33 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Leucine-Rich Repeat-Containing Protein 33, Transforming growth factor beta activator LRRC33, Negative Regulator Of Reactive Oxygen Species, NRROS
  • Anti-NALCN/VGCNL1 (extracellular) Antibody
    Anti-NALCN/VGCNL1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Sodium Leak Channel Non-Selective Protein (NALCN)
    • Product Code: ASC-022
    • Short Description: A Rabbit Polyclonal Antibody to Sodium Leak Channel Non-Selective Protein (NALCN)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Sodium leak channel non-selective protein, Voltage gated channel-like protein 1, Four domain-type voltage-gated ion channel alpha-1 subunit, CanIon, Rb21-channel
  • Anti-OCTN1/SLC22A4 Antibody
    Anti-OCTN1/SLC22A4 Antibody
    A Rabbit Polyclonal antibody to OCTN1/SLC22A4 transporter.
    • Product Code: ACT-014
    • Short Description: A Rabbit Polyclonal antibody to OCTN1/SLC22A4 transporter.
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Organic Cation/Carnitine Transporter 1, Ergothioneine Transporter, Solute Carrier Family 22 Member 4
  • Anti-Pannexin 2 (extracellular) Antibody
    Anti-Pannexin 2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Pannexin 2
    • Product Code: ACC-232
    • Short Description: A Rabbit Polyclonal Antibody to Pannexin 2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: PANX2, PX2, hPANX2
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