Neuroimmunology

Neuroimmunology

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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223 Results
  • Anti-PMCA3 Antibody
    Anti-PMCA3 Antibody
    A Rabbit Polyclonal Antibody to PMCA3 ATPase
    • Product Code: ACP-003
    • Short Description: A Rabbit Polyclonal Antibody to PMCA3 ATPase
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Plasma membrane Ca2+ ATPase 3, Plasma membrane calcium pump isoform 3, ATP2B3, SCAX1
  • Anti-Prostaglandin F2-α Receptor/PTGFR Antibody
    Anti-Prostaglandin F2-α Receptor/PTGFR Antibody
    A Rabbit Polyclonal Antibody to Prostaglandin F2-α Receptor
    • Product Code: APR-067
    • Short Description: A Rabbit Polyclonal Antibody to Prostaglandin F2-α Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Prostaglandin F2-alpha receptor, Prostaglandin F receptor, Prostanoid FP receptor, PGF2-alpha receptor
  • Anti-SCN7A (Na<sub>X</sub>) Antibody
    Anti-SCN7A (NaX) Antibody
    A Rabbit Polyclonal antibody to SCN7A (NaX)
    • Product Code: ASC-050
    • Short Description: A Rabbit Polyclonal antibody to SCN7A (NaX)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Sodium Channel Protein Type 7 Subunit Alpha, Putative voltage-gated sodium channel subunit alpha Nax, SCN6A, Nav2.1, Nav2.2
  • Anti-Semaphorin 4A (SEMA4A) (extracellular) Antibody
    Anti-Semaphorin 4A (SEMA4A) (extracellular) Antibody
    A Rabbit Polyclonal antibody to Semaphorin 4A (extracellular).
    • Product Code: ASR-065
    • Short Description: A Rabbit Polyclonal antibody to Semaphorin 4A (extracellular).
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunohistochemistry
    • Target: SEMAB
  • Anti-Semaphorin 6A (extracellular) Antibody
    Anti-Semaphorin 6A (extracellular) Antibody
    A Rabbit Polyclonal antibody to Semaphorin 6A
    • Product Code: ASR-071
    • Short Description: A Rabbit Polyclonal antibody to Semaphorin 6A
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: SEMAQ, SEMA6A-1, SEMA6A
  • Anti-SERCA3 Antibody
    Anti-SERCA3 Antibody
    A Rabbit Polyclonal Antibody to SERCA3 Pump
    • Product Code: ACP-014
    • Short Description: A Rabbit Polyclonal Antibody to SERCA3 Pump
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Sarcoplasmic/endoplasmic reticulum calcium ATPase 3, Calcium pump 3, ATP2A3
  • Anti-SLC17A5 (Sialin) Antibody
    Anti-SLC17A5 (Sialin) Antibody
    A Rabbit Polyclonal Antibody to SLC17A5 (Sialin) Transporter
    • Product Code: AST-015
    • Short Description: A Rabbit Polyclonal Antibody to SLC17A5 (Sialin) Transporter
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Sialic acid transporter, H+/sialic acid cotransporter, Vesicular H+/aspartate-glutamate cotransporter
  • Anti-SLC22A1 Antibody
    Anti-SLC22A1 Antibody
    A Rabbit Polyclonal Antibody to Organic Cation Transporter 1
    • Product Code: ACT-011
    • Short Description: A Rabbit Polyclonal Antibody to Organic Cation Transporter 1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: OCT1, Organic cation transporter 1
  • Anti-SLC22A3 (extracellular) Antibody
    Anti-SLC22A3 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Organic Cation Transporter 3
    • Product Code: ACT-013
    • Short Description: A Rabbit Polyclonal Antibody to Organic Cation Transporter 3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: OCT3, Organic cation transporter 3
  • Anti-Slitrk1 (extracellular) Antibody
    KO Validate'
    Anti-Slitrk1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Slitrk1
    • Product Code: ASR-081
    • Short Description: A Rabbit Polyclonal Antibody to Slitrk1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: SLIT and NTRK-like family member 1, Leucine-rich repeat-containing protein 12, LRRC12
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