Neurodegenerative Diseases

Neurodegenerative Diseases

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.
  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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941 Results
  • Anti-Sortilin (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Sortilin Conjugated to the Fluorescent Dye FITC
    • Product Code: ANT-009-F
    • Short Description: A Rabbit Polyclonal Antibody to Sortilin Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: Sortilin 1, SORT1, Neurotensin receptor 3, NTR3, NT3, Glycoprotein 95, Gp95
  • Anti-SPARC Antibody
    A Rabbit Polyclonal Antibody to SPARC
    • Product Code: ANR-225
    • Short Description: A Rabbit Polyclonal Antibody to SPARC
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: SPARC
  • Anti-STIM2 Antibody
    A Rabbit Polyclonal Antibody to STIM2
    • Product Code: ACC-064
    • Short Description: A Rabbit Polyclonal Antibody to STIM2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Stromal interaction molecule 2
  • Anti-SV2A Antibody
    A Rabbit Polyclonal Antibody to SV2A
    • Product Code: ANR-095
    • Short Description: A Rabbit Polyclonal Antibody to SV2A
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunoprecipitation, Immunohistochemistry
    • Target: Synaptic vesicle glycoprotein 2A, Synaptic vesicle protein 2A
  • Anti-Synapsin I (SYN1) Antibody
    A Rabbit Polyclonal Antibody to Synapsin I
    • Product Code: ANR-014
    • Short Description: A Rabbit Polyclonal Antibody to Synapsin I
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Synapsin-1
  • Anti-Synapsin II (SYN2) Antibody
    A Rabbit Polyclonal Antibody to Synapsin II
    • Product Code: ANR-015
    • Short Description: A Rabbit Polyclonal Antibody to Synapsin II
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Synapsin-2
  • Anti-Synapsin III (SYN3) Antibody
    A Rabbit Polyclonal Antibody to Synapsin III
    • Product Code: ANR-017
    • Short Description: A Rabbit Polyclonal Antibody to Synapsin III
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Synapsin-3
  • Anti-Synaptojanin 1 Antibody
    A Rabbit Polyclonal Antibody to Synaptojanin 1
    • Product Code: APZ-061
    • Short Description: A Rabbit Polyclonal Antibody to Synaptojanin 1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: SYNJ1
  • Anti-Synaptophysin Antibody
    A Rabbit Polyclonal Antibody to Synaptophysin
    • Product Code: ANR-013
    • Short Description: A Rabbit Polyclonal Antibody to Synaptophysin
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Major synaptic vesicle protein p38, SYP
  • Anti-Synaptotagmin-1 (SYT1) Antibody
    A Rabbit Polyclonal Antibody to Synaptotagmin-1
    • Product Code: ANR-003
    • Short Description: A Rabbit Polyclonal Antibody to Synaptotagmin-1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Synaptotagmin I, SytI, p65, SVP65
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