Neurodegenerative Diseases

Neurodegenerative Diseases

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.
  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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941 Results
  • Recombinant human Persephin protein
    A recombinant human neurotrophic factor that promotes the survival of dopaminergic and motor neurons, primarily via the GFRα4 and RET receptor complex
    • Product Code: P-320
    • Short Description: A recombinant human neurotrophic factor that promotes the survival of dopaminergic and motor neurons, primarily via the GFRα4 and RET receptor complex
    • Applications: Proliferation assay, Neurite outgrowth assay
    • MW: 20.6 kDa (dimer)
  • Recombinant human Pleiotrophin protein
    A recombinant human heparin-binding growth factor (PTN) that promotes neuronal growth, angiogenesis, and cell proliferation by binding to receptors including PTPRZ and ALK
    • Product Code: P-150
    • Short Description: A recombinant human heparin-binding growth factor (PTN) that promotes neuronal growth, angiogenesis, and cell proliferation by binding to receptors including PTPRZ and ALK
    • Applications: Neurite outgrowth assay
    • MW: 15.4 kDa
  • Recombinant human proBDNF (cleavage resistant) protein
    A recombinant human, cleavage-resistant proBDNF protein produced in E. coli that functions exclusively as a ligand for the p75NTR/sortilin receptor complex
    • Product Code: B-256
    • Short Description: A recombinant human, cleavage-resistant proBDNF protein produced in E. coli that functions exclusively as a ligand for the p75NTR/sortilin receptor complex
    • Applications: Cell Culture, Western blot
    • MW: 51.41 kDa (dimer)
  • Recombinant human proBDNF protein
    A recombinant human proBDNF protein produced in E. coli that functions exclusively as a ligand for the p75NTR/sortilin receptor complex
    • Product Code: B-257
    • Short Description: A recombinant human proBDNF protein produced in E. coli that functions exclusively as a ligand for the p75NTR/sortilin receptor complex
    • Applications: Cell Culture, In vitro binding assay, Western blot
    • MW: 51.78 kDa (dimer)
  • Recombinant human proNGF (cleavage resistant) protein
    A cleavage-resistant recombinant human proNGF that binds TrkA, p75NTR, and SORT1 receptors involved in both cell survival and apoptosis
    • Product Code: N-285
    • Short Description: A cleavage-resistant recombinant human proNGF that binds TrkA, p75NTR, and SORT1 receptors involved in both cell survival and apoptosis
    • Applications: Cell Culture, Survival assay, Neurite outgrowth assay
    • MW: 49.9 kDa (dimer)
  • Recombinant human proNGF protein
    A recombinant human proNGF that binds TrkA, p75NTR, and SORT1 receptors involved in both cell survival and apoptosis
    • Product Code: N-280
    • Short Description: A recombinant human proNGF that binds TrkA, p75NTR, and SORT1 receptors involved in both cell survival and apoptosis
    • Applications: Cell Culture, Survival assay, Neurite outgrowth assay
    • MW: 50.2 kDa (dimer)
  • Recombinant human Val66Met proBDNF (cleavage resistant) protein
    A cleavage-resistant human proBDNF variant (Met66) for the study of p75NTR and sortilin receptor signaling
    • Product Code: B-456
    • Short Description: A cleavage-resistant human proBDNF variant (Met66) for the study of p75NTR and sortilin receptor signaling
    • Applications: Cell Culture, Western blot
    • MW: 51.45 kDa (dimer)
  • Recombinant human Val66Met proBDNF protein
    A human proBDNF variant (Met66) for the study of p75NTR and sortilin receptor signaling
    • Product Code: B-457
    • Short Description: A human proBDNF variant (Met66) for the study of p75NTR and sortilin receptor signaling
    • Applications: Cell Culture, Western blot
    • MW: 51.45 kDa (dimer)
  • Recombinant mouse proBDNF (cleavage resistant) protein
    A recombinant mouse, cleavage-resistant proBDNF protein produced in E. coli that functions as a ligand for the p75NTR/sortilin receptor complex
    • Product Code: B-243
    • Short Description: A recombinant mouse, cleavage-resistant proBDNF protein produced in E. coli that functions as a ligand for the p75NTR/sortilin receptor complex
    • Applications: Cell Culture, In vivo, Electrophysiology, Western blot
    • MW: 52 kDa (dimer)
  • Recombinant mouse proBDNF protein
    A recombinant mouse proBDNF protein produced in E. coli that functions as a ligand for the p75NTR/sortilin receptor complex
    • Product Code: B-240
    • Short Description: A recombinant mouse proBDNF protein produced in E. coli that functions as a ligand for the p75NTR/sortilin receptor complex
    • Applications: In vitro, In vivo, Electrophysiology, Western blot
    • MW: 52.4 kDa (dimer)
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