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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

738 Results
  • Anti-GPR143 Antibody
    Anti-GPR143 Antibody
    A Rabbit Polyclonal antibody to GPR143
    • Product Code: AGR-083
    • Short Description: A Rabbit Polyclonal antibody to GPR143
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: G-Protein Coupled Receptor 143, Ocular Albinism Type 1 Protein, OA1
  • Anti-GPR158 Antibody
    Anti-GPR158 Antibody
    A Rabbit Polyclonal antibody to GPR158 receptor
    • Product Code: AGR-078
    • Short Description: A Rabbit Polyclonal antibody to GPR158 receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Probable G-protein coupled receptor 158, Metabotropic Glycine Receptor, mGlyR
  • Anti-GPR160 Antibody
    Anti-GPR160 Antibody
    A Rabbit Polyclonal antibody to GPR160
    • Product Code: AGR-090
    • Short Description: A Rabbit Polyclonal antibody to GPR160
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: G-Protein Coupled Receptor 160
  • Anti-GPR171 (extracellular) Antibody
    Anti-GPR171 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to GPR171
    • Product Code: AGR-054
    • Short Description: A Rabbit Polyclonal Antibody to GPR171
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: G-Protein Coupled Receptor 171, Platelet Activating Receptor Homolog, G-protein coupled receptor H963
  • Anti-GPR171 (extracellular)-PE Antibody
    Anti-GPR171 (extracellular)-PE Antibody
    A Rabbit Polyclonal Antibody to GPR171 (extracellular) conjugated to the fluorescent dye Phycoerythrin (PE)
    • Product Code: AGR-054-PE
    • Short Description: A Rabbit Polyclonal Antibody to GPR171 (extracellular) conjugated to the fluorescent dye Phycoerythrin (PE)
    • Host: Rabbit
    • Label: R-PE
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: G-Protein Coupled Receptor 171, Platelet Activating Receptor Homolog, G-protein coupled receptor H963
  • Anti-GPR37L1 (extracellular) Antibody
    KO Validate'
    Anti-GPR37L1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to GPR37L1 (extracellular)
    • Product Code: AGR-050
    • Short Description: A Rabbit Polyclonal Antibody to GPR37L1 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: G-Protein Coupled Receptor 37-Like 1, Endothelin B Receptor-Like Protein 2, ETBRLP2, Prosaposin Receptor GPR37L1
  • Anti-GPR50 Antibody
    Anti-GPR50 Antibody
    A Rabbit Polyclonal antibody to GPR50
    • Product Code: AGR-061
    • Short Description: A Rabbit Polyclonal antibody to GPR50
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: G Protein-Coupled Receptor 50, Melatonin-Related Receptor
  • Anti-GPR56 (extracellular) Antibody
    Anti-GPR56 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to GPR56
    • Product Code: AGR-047
    • Short Description: A Rabbit Polyclonal Antibody to GPR56
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: G-protein coupled receptor 56, Adhesion G-protein coupled receptor G1, ADGRG1
  • Anti-GPR56 (extracellular)-FITC Antibody
    Anti-GPR56 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to GPR56 Conjugated to the Fluorescent Dye FITC
    • Product Code: AGR-047-F
    • Short Description: A Rabbit Polyclonal Antibody to GPR56 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: G-protein coupled receptor 56, Adhesion G-protein coupled receptor G1, ADGRG1
  • Anti-GPR97 (ADGRG3)(extracellular) Antibody
    Anti-GPR97 (ADGRG3)(extracellular) Antibody
    A Rabbit Polyclonal antibody to GPR97 (ADGRG3) (extracellular)
    • Product Code: AGR-057
    • Short Description: A Rabbit Polyclonal antibody to GPR97 (ADGRG3) (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Adhesion G Protein-Coupled Receptor G3, G-protein coupled receptor 97
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