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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

738 Results
  • Anti-KCNK18 (TRESK) (extracellular) Antibody
    Anti-KCNK18 (TRESK) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to KCNK18 Channel
    • Product Code: APC-122
    • Short Description: A Rabbit Polyclonal Antibody to KCNK18 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Potassium channel subfamily K member 18, TWIK-related spinal cord K+ channel, K2P18.1
  • Anti-KCNK2 (TREK-1) Antibody
    KO Validate'
    Anti-KCNK2 (TREK-1) Antibody
    A Rabbit Polyclonal Antibody to KCNK2 Channel
    • Product Code: APC-047
    • Short Description: A Rabbit Polyclonal Antibody to KCNK2 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Potassium channel subfamily K member 2, Outward rectifying potassium channel protein TREK-1, Twik-related K+ channel 1, K2P2.1, TPKC1
  • Anti-KCNK4 (TRAAK) Antibody
    Anti-KCNK4 (TRAAK) Antibody
    A Rabbit Polyclonal Antibody to KCNK4 Channel
    • Product Code: APC-108
    • Short Description: A Rabbit Polyclonal Antibody to KCNK4 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Potassium channel subfamily K member 4, K2P4.1, TWIK-related arachidonic acid-stimulated K+ channel protein, Two pore K+ channel KT4.1
  • Anti-KCNK6 (TWIK-2) Antibody
    Anti-KCNK6 (TWIK-2) Antibody
    A Rabbit Polyclonal Antibody to KCNK6 Channel
    • Product Code: APC-040
    • Short Description: A Rabbit Polyclonal Antibody to KCNK6 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Mouse
    • Applications: Western blot
    • Target: Potassium channel subfamily K member 6, TOSS, K2P6.1
  • Anti-KCNK9 (TASK-3) (extracellular) Antibody
    Anti-KCNK9 (TASK-3) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to KCNK9 Channel 
    • Product Code: APC-044
    • Short Description: A Rabbit Polyclonal Antibody to KCNK9 Channel 
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Potassium channel subfamily K member 9, K2P9.1, TWIK-related acid-sensitive K+ channel 3, Two pore K+ channel KT3.2
  • Anti-KCNN1 (K<sub>Ca</sub>2.1, SK1) Antibody
    Anti-KCNN1 (KCa2.1, SK1) Antibody
    A Rabbit Polyclonal Antibody to KCNN1 (KCa2.1, SK1) Channel
    • Product Code: APC-039
    • Short Description: A Rabbit Polyclonal Antibody to KCNN1 (KCa2.1, SK1) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: SKCa1, Small conductance calcium-activated potassium channel protein 1
  • Anti-KCNS1 (K<sub>V</sub>9.1) (extracellular) Antibody
    Anti-KCNS1 (KV9.1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to KCNS1 (KV9.1) Channel
    • Product Code: APC-131
    • Short Description: A Rabbit Polyclonal Antibody to KCNS1 (KV9.1) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Potassium voltage-gated channel subfamily S member 1
  • Anti-KCNS2 (K<sub>V</sub>9.2) Antibody
    Anti-KCNS2 (KV9.2) Antibody
    A Rabbit Polyclonal Antibody to KV9.2 Channel
    • Product Code: APC-132
    • Short Description: A Rabbit Polyclonal Antibody to KV9.2 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Voltage-gated K+ channel subunit KV9.2
  • Anti-KCNS3 (K<sub>V</sub>9.3) (extracellular) Antibody
    Anti-KCNS3 (KV9.3) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to KCNS3 (KV9.3) Channel
    • Product Code: APC-133
    • Short Description: A Rabbit Polyclonal Antibody to KCNS3 (KV9.3) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Potassium voltage-gated channel subfamily S member 3
  • Anti-KCNT2 (Slick) Antibody
    Anti-KCNT2 (Slick) Antibody
    A Rabbit Polyclonal Antibody to KCNT2 (Slick)
    • Product Code: APC-126
    • Short Description: A Rabbit Polyclonal Antibody to KCNT2 (Slick)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence
    • Target: KCa4.2, Slo2.1 Na+ and Cl--activated K+ channel, Potassium channel subfamily T member 2
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