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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

738 Results
  • Anti-Kidins220 Antibody
    Anti-Kidins220 Antibody
    A Rabbit Polyclonal Antibody to Kidins220
    • Product Code: APZ-017
    • Short Description: A Rabbit Polyclonal Antibody to Kidins220
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Kinase D-interacting substrate of 220 kDa, Ankyrin repeat-rich membrane-spanning protein, ARMS
  • Anti-K<sub>ir</sub>2.2 (KCNJ12) Antibody
    Anti-Kir2.2 (KCNJ12) Antibody
    A Rabbit Polyclonal Antibody to Kir2.2 (KCNJ12) Channel
    • Product Code: APC-042
    • Short Description: A Rabbit Polyclonal Antibody to Kir2.2 (KCNJ12) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Inward rectifier K+ channel Kir2.2, IRK-2, IRK2, ATP-sensitive inward rectifier potassium channel 12
  • Anti-K<sub>ir</sub>5.1 Antibody
    Anti-Kir5.1 Antibody
    A Rabbit Polyclonal Antibody to Kir5.1 Channel
    • Product Code: APC-123
    • Short Description: A Rabbit Polyclonal Antibody to Kir5.1 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Inward rectifier potassium channel 16, KCNJ16, BIR9
  • Anti-K<sub>ir</sub>7.1 (extracellular) Antibody
    Anti-Kir7.1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Kir7.1 Channel
    • Product Code: APC-125
    • Short Description: A Rabbit Polyclonal Antibody to Kir7.1 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunohistochemistry
    • Target: Inward rectifier potassium channel 13, KCNJ13
  • Anti-K<sub>ir</sub>7.1 (extracellular)-ATTO Fluor-488 Antibody
    Anti-Kir7.1 (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to Kir7.1 Channel Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: APC-125-AG
    • Short Description: A Rabbit Polyclonal Antibody to Kir7.1 Channel Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Inward rectifier potassium channel 13, KCNJ13
  • Anti-K<sub>V</sub>1.3 (KCNA3) (extracellular) Antibody
    KO Validate'
    Anti-KV1.3 (KCNA3) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to KV1.3 (KCNA3) Channel
    • Product Code: APC-101
    • Short Description: A Rabbit Polyclonal Antibody to KV1.3 (KCNA3) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Potassium voltage-gated channel subfamily A member 3
  • Anti-K<sub>V</sub>1.3 (KCNA3) (extracellular)-Biotin Antibody
    Anti-KV1.3 (KCNA3) (extracellular)-Biotin Antibody
    A Rabbit Polyclonal Antibody to KV1.3 (KCNA3) Channel Conjugated to Biotin 
    • Product Code: APC-101-B
    • Short Description: A Rabbit Polyclonal Antibody to KV1.3 (KCNA3) Channel Conjugated to Biotin 
    • Host: Rabbit
    • Label: Biotin
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Flow cytometry, Immunohistochemistry
    • Target: Potassium voltage-gated channel subfamily A member 3
  • Anti-K<sub>V</sub>1.3 (KCNA3) (extracellular)-FITC Antibody
    Anti-KV1.3 (KCNA3) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to KV1.3 (KCNA3) Channel Conjugated to the Fluorescent Dye FITC
    • Product Code: APC-101-F
    • Short Description: A Rabbit Polyclonal Antibody to KV1.3 (KCNA3) Channel Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Immunocytochemistry, Immunofluorescence, Flow cytometry
    • Target: Potassium voltage-gated channel subfamily A member 3
  • Anti-K<sub>V</sub>1.3 (KCNA3) (extracellular)-PE Antibody
    Anti-KV1.3 (KCNA3) (extracellular)-PE Antibody
    A Rabbit Polyclonal Antibody to KV1.3 (KCNA3) Channel Conjugated to the Fluorescent Dye Phycoerythrin
    • Product Code: APC-101-PE
    • Short Description: A Rabbit Polyclonal Antibody to KV1.3 (KCNA3) Channel Conjugated to the Fluorescent Dye Phycoerythrin
    • Host: Rabbit
    • Label: R-PE
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Potassium voltage-gated channel subfamily A member 3
  • Anti-K<sub>V</sub>1.4 (extracellular) Antibody
    Anti-KV1.4 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to KV1.4 Channel
    • Product Code: APC-167
    • Short Description: A Rabbit Polyclonal Antibody to KV1.4 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Potassium voltage-gated channel subfamily A member 4, KCNA4
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