Neurodevelopment

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

738 Results
  • Anti-Nogo Receptor (extracellular) Antibody
    Anti-Nogo Receptor (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Nogo Receptor
    • Product Code: ANT-008
    • Short Description: A Rabbit Polyclonal Antibody to Nogo Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: NogoR, Nogo-R, NgR, NgR1, Nogo-66 receptor, Reticulon 4 receptor, RTN4R
  • Anti-Nogo Receptor (extracellular)-FITC Antibody
    Anti-Nogo Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Nogo Receptor Conjugated to the Fluorescent Dye FITC
    • Product Code: ANT-008-F
    • Short Description: A Rabbit Polyclonal Antibody to Nogo Receptor Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: NogoR, Nogo-R, NgR, NgR1, Nogo-66 receptor, Reticulon 4 receptor, RTN4R
  • Anti-NrCAM (extracellular) Antibody
    Anti-NrCAM (extracellular) Antibody
    A Rabbit Polyclonal Antibody to NrCAM
    • Product Code: ANR-045
    • Short Description: A Rabbit Polyclonal Antibody to NrCAM
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Neuronal cell adhesion molecule, Nr-CAM, NgCAM-related cell adhesion molecule, Ankyrin-binding cell adhesion molecule, Neuronal surface protein Bravo, HBravo
  • Anti-NrCAM (extracellular)-FITC Antibody
    Anti-NrCAM (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to NrCAM (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ANR-045-F
    • Short Description: A Rabbit Polyclonal Antibody to NrCAM (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Neuronal cell adhesion molecule, Nr-CAM, NgCAM-related cell adhesion molecule, Ankyrin-binding cell adhesion molecule, Neuronal surface protein Bravo, HBravo
  • Anti-NRG2 (extracellular) Antibody
    Anti-NRG2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Neuregulin-2
    • Product Code: ANR-114
    • Short Description: A Rabbit Polyclonal Antibody to Neuregulin-2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Neuregulin-2, Pro-neuregulin-2 membrane-bound isoform, Pro-NRG2, Neural- and thymus-derived activator for ErbB kinases, NTAK
  • Anti-NRG3 (extracellular) Antibody
    Anti-NRG3 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Neuregulin-3
    • Product Code: ANR-115
    • Short Description: A Rabbit Polyclonal Antibody to Neuregulin-3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Neuregulin-3, HRG3
  • Anti-OCTN2/SLC22A5 Antibody
    Anti-OCTN2/SLC22A5 Antibody
    A rabbit polyclonal antibody to OCTN2/SLC22A5
    • Product Code: ACT-015
    • Short Description: A rabbit polyclonal antibody to OCTN2/SLC22A5
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: SLC22A5
  • Anti-Opsin 3 (extracellular) Antibody
    Anti-Opsin 3 (extracellular) Antibody
    A Rabbit Polyclonal antibody to Opsin 3 (extracellular)
    • Product Code: AOR-023
    • Short Description: A Rabbit Polyclonal antibody to Opsin 3 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: OPN3, Encephalopsin, Panopsin
  • Anti-Orexin Receptor 1 Antibody
    Anti-Orexin Receptor 1 Antibody
    A Rabbit Polyclonal Antibody to Orexin Receptor 1
    • Product Code: AOR-001
    • Short Description: A Rabbit Polyclonal Antibody to Orexin Receptor 1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: OX1R, Hypocretin receptor type 1, HCRTR1
  • Anti-Orexin Receptor 1-ATTO Fluor-488 Antibody
    Anti-Orexin Receptor 1-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to Orexin Receptor 1 Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: AOR-001-AG
    • Short Description: A Rabbit Polyclonal Antibody to Orexin Receptor 1 Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: OX1R, Hypocretin receptor type 1, HCRTR1
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