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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

738 Results
  • Ala<sup>5</sup>-Galanin (2-11)
    Bioassay Tested
    Ala5-Galanin (2-11)
    A potent agonist selective for the GALR2 Galanin receptors
    • Product Code: GPG-300
    • Short Description: A potent agonist selective for the GALR2 Galanin receptors
    • Applications: Aequorin functional assay, Calcium imaging assay
    • MW: 1120 Da
  • ANA-12
    Bioassay Tested
    ANA-12
    A Selective Antagonist of TrkB Receptor
    • Product Code: A-215
    • Short Description: A Selective Antagonist of TrkB Receptor
    • CAS No.: 219766-25-3
    • MW: 407.49
  • Anti-5HT5B Receptor (HTR5B) (extracellular) Antibody
    Anti-5HT5B Receptor (HTR5B) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 5B
    • Product Code: ASR-040
    • Short Description: A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 5B
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: 5-Hydroxytryptamine receptor 5B, Serotonin receptor 5B, MR22
  • Anti-Aquaporin 6 Antibody
    Anti-Aquaporin 6 Antibody
    A Rabbit Polyclonal Antibody to Aquaporin 6 Channel
    • Product Code: AQP-006
    • Short Description: A Rabbit Polyclonal Antibody to Aquaporin 6 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: AQP6, AQP2L, Kidney-specific aquaporin
  • Anti-ASIC4 Antibody
    Anti-ASIC4 Antibody
    A Rabbit Polyclonal Antibody to ASIC4 Channel
    • Product Code: ASC-015
    • Short Description: A Rabbit Polyclonal Antibody to ASIC4 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Acid-sensing ion channel 4, H+-gated cation channel, Amiloride-sensitive cation channel 4, BNaC4, SPASIC, ACCN4
  • Anti-ASTN1 (extracellular) Antibody
    Anti-ASTN1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Astrotactin-1
    • Product Code: ANR-151
    • Short Description: A Rabbit Polyclonal Antibody to Astrotactin-1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Astrotactin-1
  • Anti-ATP1B3 (extracellular) Antibody
    Anti-ATP1B3 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Beta 3 Na+/K+ ATPase
    • Product Code: ANP-013
    • Short Description: A Rabbit Polyclonal Antibody to Beta 3 Na+/K+ ATPase
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunohistochemistry
    • Target: Sodium/Potassium-Transporting ATPase Subunit Beta-3, CD298, Beta 3 Na+/K+ ATPase
  • Anti-Beta 1 Na<sup>+</sup>/K<sup>+</sup> ATPase (extracellular) Antibody
    Anti-Beta 1 Na+/K+ ATPase (extracellular) Antibody
    A Rabbit Polyclonal antibody to Beta 1 Na+/K+ ATPase (extracellular)
    • Product Code: ANP-014
    • Short Description: A Rabbit Polyclonal antibody to Beta 1 Na+/K+ ATPase (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Sodium-Potassium ATPase Subunit Beta 1, ATP1B, ATP1B1
  • Anti-Beta 2 Na<sup>+</sup>/K<sup>+</sup> ATPase (extracellular) Antibody
    Anti-Beta 2 Na+/K+ ATPase (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Beta 2 Na+/K+ ATPase
    • Product Code: ANP-012
    • Short Description: A Rabbit Polyclonal Antibody to Beta 2 Na+/K+ ATPase
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: ATP1B2, Sodium/potassium-transporting ATPase subunit beta-2, Adhesion molecule on glia, AMOG
  • Anti-Beta 2 Na+/K+ ATPase (extracellular)-FITC Antibody
    Anti-Beta 2 Na+/K+ ATPase (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to ?-Opioid Receptor (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ANP-012-F
    • Short Description: A Rabbit Polyclonal Antibody to ?-Opioid Receptor (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: ATP1B2, Sodium/potassium-transporting ATPase subunit beta-2, Adhesion molecule on glia, AMOG
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