Neurodevelopment

Neurodevelopment

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

738 Results
  • Anti-TMC1 (extracellular) Antibody
    Anti-TMC1 (extracellular) Antibody
    A Rabbit Polyclonal antibody to TMC1 (extracellular) 
    • Product Code: ATC-010
    • Short Description: A Rabbit Polyclonal antibody to TMC1 (extracellular) 
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: Transmembrane Channel Like 1, Transmembrane cochlear-expressed protein 1
  • Anti-TMEM108 (extracellular) Antibody
    Anti-TMEM108 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to TMEM108 (Extracellular)
    • Product Code: ANR-228
    • Short Description: A Rabbit Polyclonal Antibody to TMEM108 (Extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: TMEM108
  • Anti-TMEM175 Antibody
    Anti-TMEM175 Antibody
    A Rabbit Polyclonal antibody to TMEM175
    • Product Code: APC-175
    • Short Description: A Rabbit Polyclonal antibody to TMEM175
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Transmembrane Protein 175, Endosomal/Lysosomal Proton Channel TMEM175
  • Anti-TMEM66 Antibody
    Anti-TMEM66 Antibody
    A Rabbit Polyclonal Antibody to TMEM66
    • Product Code: ACC-067
    • Short Description: A Rabbit Polyclonal Antibody to TMEM66
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Store-operated calcium entry-associated regulatory factor, SOCE-associated regulatory factor, SARAF, Transmembrane protein 66
  • Anti-TMEM66-ATTO Fluor-594 Antibody
    Anti-TMEM66-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to TMEM66 Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ACC-067-AR
    • Short Description: A Rabbit Polyclonal Antibody to TMEM66 Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Store-operated calcium entry-associated regulatory factor, SOCE-associated regulatory factor, SARAF, Transmembrane protein 66
  • Anti-TRIC-A (TMEM38A) Antibody
    Anti-TRIC-A (TMEM38A) Antibody
    A Rabbit Polyclonal Antibody to TRIC-A Channel
    • Product Code: ATC-002
    • Short Description: A Rabbit Polyclonal Antibody to TRIC-A Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunohistochemistry
    • Target: Trimeric intracellular cation channel type A, 27 kDa sarcoplasmic reticulum protein, Transmembrane protein 38A
  • Anti-TRIC-B (TMEM38B) Antibody
    Anti-TRIC-B (TMEM38B) Antibody
    A Rabbit Polyclonal Antibody to TRIC-B Channel
    • Product Code: ATC-001
    • Short Description: A Rabbit Polyclonal Antibody to TRIC-B Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunohistochemistry
    • Target: Trimeric intracellular cation channel type B, Transmembrane protein 38B
  • Anti-TrkA (extracellular) Antibody
    KO Validate'
    Anti-TrkA (extracellular) Antibody
    A Rabbit Polyclonal Antibody to TrkA
    • Product Code: ANT-018
    • Short Description: A Rabbit Polyclonal Antibody to TrkA
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: High affinity nerve growth factor receptor, Neurotrophic tyrosine kinase receptor type 1, NTRK1
  • Anti-TrkA (extracellular)-ATTO Fluor-633 Antibody
    Anti-TrkA (extracellular)-ATTO Fluor-633 Antibody
    A Rabbit Polyclonal Antibody to TrkA Conjugated to the Fluorescent Dye ATTO-633
    • Product Code: ANT-018-FR
    • Short Description: A Rabbit Polyclonal Antibody to TrkA Conjugated to the Fluorescent Dye ATTO-633
    • Host: Rabbit
    • Label: ATTO 633
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: High affinity nerve growth factor receptor, Neurotrophic tyrosine kinase receptor type 1, NTRK1
  • Anti-TrkA (extracellular)-FITC Antibody
    Anti-TrkA (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to TrkA Conjugated to the Fluorescent Dye FITC
    • Product Code: ANT-018-F
    • Short Description: A Rabbit Polyclonal Antibody to TrkA Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: High affinity nerve growth factor receptor, Neurotrophic tyrosine kinase receptor type 1, NTRK1
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