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Neurodevelopment

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.
  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

738 Results
  • Anti-CatSper1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CatSper1 Channel
    • Product Code: ACC-301
    • Short Description: A Rabbit Polyclonal Antibody to CatSper1 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: Cation channel sperm-associated protein 1
  • Anti-CatSper4 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CatSper4 Channel
    • Product Code: ACC-304
    • Short Description: A Rabbit Polyclonal Antibody to CatSper4 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence
    • Target: Cation channel sperm-associated protein 4
  • Anti-CHERP Antibody
    A Rabbit Polyclonal Antibody to CHERP
    • Product Code: ACC-330
    • Short Description: A Rabbit Polyclonal Antibody to CHERP
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Calcium homeostasis endoplasmic reticulum protein, SR-related CTD-associated factor 6, SCAF6
  • Anti-CHERP-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to CHERP Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ACC-330-AR
    • Short Description: A Rabbit Polyclonal Antibody to CHERP Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Calcium homeostasis endoplasmic reticulum protein, SR-related CTD-associated factor 6, SCAF6
  • Anti-Choline Transporter (SLC5A7) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Choline Transporter (SLC5A7)
    • Product Code: ACT-001
    • Short Description: A Rabbit Polyclonal Antibody to Choline Transporter (SLC5A7)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Na+/Cl--Dependent high affinity choline transporter 1, Hemicholinium-3-sensitive choline transporter, CHT, CHT1
  • Anti-CHRM4 Antibody
    A Rabbit Polyclonal Antibody to Muscarinic Acetylcholine Receptor M4
    • Product Code: AMR-004
    • Short Description: A Rabbit Polyclonal Antibody to Muscarinic Acetylcholine Receptor M4
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Muscarinic acetylcholine receptor M4, Cholinergic receptor muscarinic 4, mAChR M4, HM4
  • Anti-CHRM5 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Muscarinic Acetylcholine Receptor M5
    • Product Code: AMR-005
    • Short Description: A Rabbit Polyclonal Antibody to Muscarinic Acetylcholine Receptor M5
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: Muscarinic acetylcholine receptor M5, Cholinergic receptor muscarinic 5, mAChR M5, HM5
  • Anti-CNGA1 Antibody
    A Rabbit Polyclonal Antibody to CNGA1 Channel
    • Product Code: APC-071
    • Short Description: A Rabbit Polyclonal Antibody to CNGA1 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Mouse
    • Applications: Western blot
    • Target: Cyclic nucleotide-gated cation channel 1, CNG channel alpha-1, CNG1, CNCG1, Rod photoreceptor cGMP-gated channel subunit alpha, RCNC1, RP49
  • Anti-CNGA2 Antibody
    A Rabbit Polyclonal Antibody to CNGA2 Channel
    • Product Code: APC-045
    • Short Description: A Rabbit Polyclonal Antibody to CNGA2 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Cyclic nucleotide-gated cation channel 2, CNG channel alpha-2, CNG2, CNCG2, Olfactory cyclic nucleotide gated channel 1, OCNC1
  • Anti-CNGA3 Antibody
    A Rabbit Polyclonal Antibody to CNGA3 Channel
    • Product Code: APC-060
    • Short Description: A Rabbit Polyclonal Antibody to CNGA3 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Cyclic nucleotide-gated cation channel alpha 3, CNG channel alpha 3, CNG3, CNCG3, CNGα2, Cone photoreceptor cGMP-gated channel subunit alpha, CCNC1, ACHM2
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