Neurodevelopment

Neurodevelopment

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.
  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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738 Results
  • Anti-GPRC5B Antibody
    A Rabbit Polyclonal antibody to GPRC5B receptor
    • Product Code: AGR-072
    • Short Description: A Rabbit Polyclonal antibody to GPRC5B receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: G-Protein Coupled Receptor Family C Group 5 Member B, Retinoic Acid-Induced Gene 2 Protein, RAIG2
  • Anti-GRIK3 (GluK3) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Kainate Receptor GluK3
    • Product Code: AGC-040
    • Short Description: A Rabbit Polyclonal Antibody to Kainate Receptor GluK3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Glutamate receptor ionotropic kainate 3, Glutamate receptor 7, GluR7
  • Anti-GRIK5 (GluK5) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Kainate Receptor GluK5
    • Product Code: AGC-042
    • Short Description: A Rabbit Polyclonal Antibody to Kainate Receptor GluK5
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Glutamate receptor ionotropic kainate 5, Glutamate receptor KA-2, KA2
  • Anti-GSG1L Antibody
    A Rabbit Polyclonal Antibody to GSG1L
    • Product Code: AGC-048
    • Short Description: A Rabbit Polyclonal Antibody to GSG1L
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Germ cell-specific gene 1-like protein, GSG1-like protein
  • Anti-HCN3 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to HCN3 Channel
    • Product Code: APC-083
    • Short Description: A Rabbit Polyclonal Antibody to HCN3 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: K+/Na+ Hyperpolarization-activated cyclic nucleotide-gated channel 3, BCNG4, Hac3
  • Anti-HCN3 Antibody
    A Rabbit Polyclonal Antibody to HCN3 Channel
    • Product Code: APC-057
    • Short Description: A Rabbit Polyclonal Antibody to HCN3 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: K+/Na+ Hyperpolarization-activated cyclic nucleotide-gated channel 3, BCNG4, Hac3
  • Anti-Histamine H<sub>1</sub> Receptor (HRH1) Antibody
    A Rabbit Polyclonal Antibody to Histamine H1 Receptor
    • Product Code: AHR-001
    • Short Description: A Rabbit Polyclonal Antibody to Histamine H1 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: H1R, HH1R
  • Anti-Histamine H<sub>3</sub> Receptor (HRH3) Antibody
    A Rabbit Polyclonal Antibody to Histamine H3 Receptor
    • Product Code: AHR-003
    • Short Description: A Rabbit Polyclonal Antibody to Histamine H3 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: H3R, HH3R, GPCR97
  • Anti-Human P2Y11 Receptor Antibody
    A Rabbit Polyclonal Antibody to P2Y11 Receptor
    • Product Code: APR-015
    • Short Description: A Rabbit Polyclonal Antibody to P2Y11 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
    • Target: P2Y purinoceptor 11, P2RY11
  • Anti-Human ZACN (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Zinc-Activated Ion Channel
    • Product Code: AZC-001
    • Short Description: A Rabbit Polyclonal Antibody to Zinc-Activated Ion Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: Zinc-activated ligand-gated ion channel, Ligand-gated ion channel zinc-activated 1, Ligand-gated ion-channel receptor L2, ZAC, LGICZ1
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