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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

738 Results
  • Anti-MCT2 Antibody
    KO Validate'
    Anti-MCT2 Antibody
    A Rabbit Polyclonal Antibody to Monocarboxylate Transporter 2
    • Product Code: AMT-012
    • Short Description: A Rabbit Polyclonal Antibody to Monocarboxylate Transporter 2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Monocarboxylate transporter 2, Monocarboxylic acid transporter 2, SLC16A7
  • Anti-MCT3 Antibody
    Anti-MCT3 Antibody
    A Rabbit Polyclonal Antibody to Monocarboxylate Transporter 3
    • Product Code: AMT-013
    • Short Description: A Rabbit Polyclonal Antibody to Monocarboxylate Transporter 3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: Monocarboxylate transporter 3, Monocarboxylic acid transporter 3, SLC16A8
  • Anti-mGluR6 (extracellular) Antibody
    Anti-mGluR6 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 6
    • Product Code: AGC-026
    • Short Description: A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 6
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Metabotropic glutamate receptor 6, GRM6, mGlu6, Gprc1f
  • Anti-MMGT1 Antibody
    Anti-MMGT1 Antibody
    A Rabbit Polyclonal Antibody to Membrane Magnesium Transporter MMgT1
    • Product Code: ANT-149
    • Short Description: A Rabbit Polyclonal Antibody to Membrane Magnesium Transporter MMgT1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: Membrane magnesium transporter 1, ER membrane protein complex subunit 5, Transmembrane protein 32, Emc5, Tmem32
  • Anti-Mouse K<sub>V</sub>1.7 (KCNA7) Antibody
    Anti-Mouse KV1.7 (KCNA7) Antibody
    A Rabbit Polyclonal Antibody to KV1.7 (KCNA7) Channel
    • Product Code: APC-063
    • Short Description: A Rabbit Polyclonal Antibody to KV1.7 (KCNA7) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat
    • Applications: Western blot
    • Target: Potassium voltage-gated channel subfamily A member 7
  • Anti-Na<sup>+</sup>/H<sup>+</sup> Exchanger 1 (NHE-1) (extracellular) Antibody
    Anti-Na+/H+ Exchanger 1 (NHE-1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Na+/H+ Exchanger 1 (NHE-1)
    • Product Code: ANX-010
    • Short Description: A Rabbit Polyclonal Antibody to Na+/H+ Exchanger 1 (NHE-1)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Sodium/hydrogen exchanger 1, SLC9A1, APNH
  • Anti-Na<sup>+</sup>/H<sup>+</sup> Exchanger 2 (NHE-2) Antibody
    Anti-Na+/H+ Exchanger 2 (NHE-2) Antibody
    A Rabbit Polyclonal Antibody to Na+/H+ Exchanger 2 (NHE-2)
    • Product Code: ANX-002
    • Short Description: A Rabbit Polyclonal Antibody to Na+/H+ Exchanger 2 (NHE-2)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Sodium/hydrogen exchanger 2, SLC9A2
  • Anti-Na<sup>+</sup>/H<sup>+</sup> Exchanger 4 (NHE-4) Antibody
    Anti-Na+/H+ Exchanger 4 (NHE-4) Antibody
    A Rabbit Polyclonal Antibody to Na+/H+ Exchanger 4 (NHE-4)
    • Product Code: ANX-004
    • Short Description: A Rabbit Polyclonal Antibody to Na+/H+ Exchanger 4 (NHE-4)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Sodium/Hydrogen Exchanger 4, NHE4, Solute carrier family 9 member 4, SLC9A4
  • Anti-Na<sup>+</sup>/H<sup>+</sup> Exchanger 5 (NHE-5) Antibody
    Anti-Na+/H+ Exchanger 5 (NHE-5) Antibody
    A Rabbit Polyclonal Antibody to Na+/H+ Exchanger 5 (NHE-5)
    • Product Code: ANX-005
    • Short Description: A Rabbit Polyclonal Antibody to Na+/H+ Exchanger 5 (NHE-5)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Sodium/hydrogen exchanger 5, SLC9A5
  • Anti-Na<sup>+</sup>/H<sup>+</sup> Exchanger 6 (NHE-6) Antibody
    Anti-Na+/H+ Exchanger 6 (NHE-6) Antibody
    A Rabbit Polyclonal Antibody to Na+/H+ Exchanger 6 (NHE-6)
    • Product Code: ANX-006
    • Short Description: A Rabbit Polyclonal Antibody to Na+/H+ Exchanger 6 (NHE-6)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Sodium/hydrogen exchanger 6, NHE6, SLC9A6
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