Neurodevelopment

Neurodevelopment

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

738 Results
  • Anti-P2Y6 Receptor (extracellular)-PE Antibody
    Anti-P2Y6 Receptor (extracellular)-PE Antibody
    A Rabbit Polyclonal Antibody to P2Y6 Receptor (extracellular) conjugated to the fluorescent dye R-Phycoerythrin (R-PE)
    • Product Code: APR-106-PE
    • Short Description: A Rabbit Polyclonal Antibody to P2Y6 Receptor (extracellular) conjugated to the fluorescent dye R-Phycoerythrin (R-PE)
    • Host: Rabbit
    • Label: R-PE
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: P2Y purinoceptor 6, P2RY6
  • Anti-P2Y6 Receptor Antibody
    KO Validate'
    Anti-P2Y6 Receptor Antibody
    A Rabbit Polyclonal Antibody to P2Y6 Receptor
    • Product Code: APR-011
    • Short Description: A Rabbit Polyclonal Antibody to P2Y6 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: P2Y purinoceptor 6, P2RY6
  • Anti-PACSIN1 Antibody
    Anti-PACSIN1 Antibody
    A Rabbit Polyclonal Antibody to PACSIN1
    • Product Code: AIP-023
    • Short Description: A Rabbit Polyclonal Antibody to PACSIN1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Syndapin-1, Synaptic, Dynamin-associated protein I, Protein kinase C and casein kinase substrate in neurons protein 1
  • Anti-PACSIN3 Antibody
    Anti-PACSIN3 Antibody
    A Rabbit Polyclonal Antibody to PACSIN 3
    • Product Code: AIP-015
    • Short Description: A Rabbit Polyclonal Antibody to PACSIN 3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Protein kinase C and casein kinase II substrate protein 3, SH3 domain-containing protein 6511, Syndapin III, SDPIII
  • Anti-Pannexin 2 (extracellular) Antibody
    Anti-Pannexin 2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Pannexin 2
    • Product Code: ACC-232
    • Short Description: A Rabbit Polyclonal Antibody to Pannexin 2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: PANX2, PX2, hPANX2
  • Anti-Pannexin 3 Antibody
    Anti-Pannexin 3 Antibody
    A Rabbit Polyclonal Antibody to Pannexin 3
    • Product Code: ACC-233
    • Short Description: A Rabbit Polyclonal Antibody to Pannexin 3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: PANX3, PX3
  • Anti-PepT2/SLC15A2 (extracellular) Antibody
    Anti-PepT2/SLC15A2 (extracellular) Antibody
    A Rabbit Polyclonal antibody to PepT2/SLC15A2 (extracellular)
    • Product Code: APT-002
    • Short Description: A Rabbit Polyclonal antibody to PepT2/SLC15A2 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Peptide Transporter 2, Solute Carrier Family 15 Member 2, Oligopeptide Transporter - Kidney Isoform, Kidney H+/Peptide Cotransporter
  • Anti-PEX5L (TRIP8b) Antibody
    KO Validate'
    Anti-PEX5L (TRIP8b) Antibody
    A Rabbit Polyclonal Antibody to PEX5L (TRIP8b)
    • Product Code: APR-070
    • Short Description: A Rabbit Polyclonal Antibody to PEX5L (TRIP8b)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: PEX5R, PEX5Rp, PEX5-related protein, PEX5-like protein, Peroxin-5-related protein, PEX2-related protein, PXR2, Peroxisome biogenesis factor 5-like, TPR-containing Rab8b-interacting protein
  • Anti-Plexin-A4 (extracellular) Antibody
    Anti-Plexin-A4 (extracellular) Antibody
    A Rabbit Polyclonal antibody to Plexin-A4 (extracellular)
    • Product Code: APR-084
    • Short Description: A Rabbit Polyclonal antibody to Plexin-A4 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: PLXNA4
  • Anti-Plexin-B1 (extracellular) Antibody
    Anti-Plexin-B1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Plexin-B1 (extracellular)
    • Product Code: APR-088
    • Short Description: A Rabbit Polyclonal Antibody to Plexin-B1 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunohistochemistry
    • Target: PLXNB1
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