Neuroimmunology

Neuroimmunology

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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223 Results
  • Anti-Connexin-31 (GJB3) Antibody
    Anti-Connexin-31 (GJB3) Antibody
    A Rabbit Polyclonal Antibody to Connexin-31
    • Product Code: ACC-213
    • Short Description: A Rabbit Polyclonal Antibody to Connexin-31
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: CX31, Gap junction β3 protein
  • Anti-Connexin-47 (GJC2) Antibody
    Anti-Connexin-47 (GJC2) Antibody
    A Rabbit Polyclonal antibody to Connexin-47 (GJC2)
    • Product Code: ACC-222
    • Short Description: A Rabbit Polyclonal antibody to Connexin-47 (GJC2)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: CX47, Gap Junction Gamma-2 Protein, Gap Junction Alpha-12 Protein, GJA12, Connexin-46.6
  • Anti-CRTH2/GPR44 (extracellular) Antibody
    Anti-CRTH2/GPR44 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Prostanoid DP2 Receptor (CRTH2, GPR44)
    • Product Code: APR-062
    • Short Description: A Rabbit Polyclonal Antibody to Prostanoid DP2 Receptor (CRTH2, GPR44)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Prostanoid DP2 receptor, Prostaglandin D2 receptor 2, PTGDR2, CD294
  • Anti-EphA6 (extracellular) Antibody
    Anti-EphA6 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to EphA6 Receptor
    • Product Code: AER-016
    • Short Description: A Rabbit Polyclonal Antibody to EphA6 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Ephrin type-A receptor 6, EPH receptor A6, EPH homology kinase 2, EHK-2
  • Anti-Ephrin-A5 (extracellular) Antibody
    Anti-Ephrin-A5 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Ephrin A5
    • Product Code: AER-035
    • Short Description: A Rabbit Polyclonal Antibody to Ephrin A5
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: EPH-related receptor tyrosine kinase ligand 7, LERK7, AL-1, EFNA5
  • Anti-Ephrin-B1 (extracellular) Antibody
    Anti-Ephrin-B1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Ephrin-B1
    • Product Code: AER-041
    • Short Description: A Rabbit Polyclonal Antibody to Ephrin-B1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: EPH-related receptor tyrosine kinase ligand 2, LERK2, EFNB1
  • Anti-Ephrin-B2 (extracellular) Antibody
    Anti-Ephrin-B2 (extracellular) Antibody
    A Rabbit Polyclonal antibody to Ephrin-B2 (extracellular)
    • Product Code: AER-042
    • Short Description: A Rabbit Polyclonal antibody to Ephrin-B2 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: EFNB2, EPH-Related Receptor Tyrosine Kinase Ligand 5, LERK5, HTK ligand, HTKL
  • Anti-GPR132 (extracellular) Antibody
    Anti-GPR132 (extracellular) Antibody
    A Rabbit Polyclonal antibody to GPR132 (extracellular)
    • Product Code: AGR-036
    • Short Description: A Rabbit Polyclonal antibody to GPR132 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: G Protein-Coupled Receptor 132, G2 Accumulation Protein, G2A
  • Anti-GPR139 Antibody
    Anti-GPR139 Antibody
    A Rabbit Polyclonal antibody to Parkin
    • Product Code: AGR-069
    • Short Description: A Rabbit Polyclonal antibody to Parkin
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: G Protein-Coupled Receptor 139, PGR3, G(Q)-Coupled Orphan Receptor GPRg1, GPRG1
  • Anti-GPR34 (extracellular) Antibody
    Anti-GPR34 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to GPR34
    • Product Code: AGR-055
    • Short Description: A Rabbit Polyclonal Antibody to GPR34
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot
    • Target: G-protein coupled receptor 34, LYPSR1
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