Neuroinflammation

Neuroinflammation

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.
  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

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123 Results
  • 17-AAG
    An Anticancer Antibiotic
    • Product Code: A-580
    • Short Description: An Anticancer Antibiotic
    • CAS No.: 75747-14-7
    • MW: 585.7
  • Aa1 Toxin
    A potent and selective blocker of KV4 (A-type) voltage-gated potassium channels
    • Product Code: RTA-400
    • Short Description: A potent and selective blocker of KV4 (A-type) voltage-gated potassium channels
    • Applications: Electrophysiology
    • MW: 3868 Da
  • Aam-KTX
    A blocker of voltage-gated potassium (KV) channels, particularly KV1.3
    • Product Code: STA-110
    • Short Description: A blocker of voltage-gated potassium (KV) channels, particularly KV1.3
    • Applications: Electrophysiology
    • MW: 4185 Da
  • ADWX-1
    A potent and specific blocker of KV1.3 channels
    • Product Code: STW-100
    • Short Description: A potent and specific blocker of KV1.3 channels
    • Applications: Electrophysiology
    • MW: 4072 Da
  • Agitoxin-1
    A potent blocker of KV1.3, KV1.1, and KV1.6 voltage-gated potassium channels
    • Product Code: STA-150
    • Short Description: A potent blocker of KV1.3, KV1.1, and KV1.6 voltage-gated potassium channels
    • Applications: Electrophysiology
    • CAS No.: 155646-21-2
    • MW: 4015 Da
  • Agitoxin-2
    A potent blocker of KV1.3, KV1.1, and KV1.6 voltage-gated potassium channels
    • Product Code: STA-420
    • Short Description: A potent blocker of KV1.3, KV1.1, and KV1.6 voltage-gated potassium channels
    • Applications: Electrophysiology
    • CAS No.: 168147-41-9
    • MW: 4091 Da
  • Agitoxin-2-Cys-TAMRA
    A fluorescent probe for imaging and sensitive detection of KV1.3, KV1.1, and KV1.6 voltage-gated potassium channels
    • Product Code: RTA-420-T
    • Short Description: A fluorescent probe for imaging and sensitive detection of KV1.3, KV1.1, and KV1.6 voltage-gated potassium channels
    • Label: TAMRA
    • Applications: Electrophysiology, Fluorescence staining
    • MW: 4673 Da
  • Agitoxin-3
    A potent blocker of KV1.3, KV1.1, and KV1.6 voltage-gated potassium channels
    • Product Code: STA-390
    • Short Description: A potent blocker of KV1.3, KV1.1, and KV1.6 voltage-gated potassium channels
    • Applications: Electrophysiology
    • CAS No.: 155646-23-4
    • MW: 4101 Da
  • Anti-Aquaporin 4 (AQP4) (249-323) Antibody
    A Rabbit Polyclonal Antibody to Aquaporin 4 (249-323) Channel
    • Product Code: AQP-004
    • Short Description: A Rabbit Polyclonal Antibody to Aquaporin 4 (249-323) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: WCH4, Mercurial-insensitive water channel
  • Anti-Aquaporin 4 (AQP4) (300-314) Antibody
    A Rabbit Polyclonal Antibody to Aquaporin 4 Channel
    • Product Code: AQP-014
    • Short Description: A Rabbit Polyclonal Antibody to Aquaporin 4 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: WCH4, Mercurial-insensitive water channel
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