Neuroinflammation

Neuroinflammation

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

123 Results
  • Anti-GPR65 (TDAG8) (extracellular)-PE Antibody
    Anti-GPR65 (TDAG8) (extracellular)-PE Antibody
    A Rabbit Polyclonal Antibody to GPR65 Conjugated to the Fluorescent Dye Phycoerythrin
    • Product Code: AGR-043-PE
    • Short Description: A Rabbit Polyclonal Antibody to GPR65 Conjugated to the Fluorescent Dye Phycoerythrin
    • Host: Rabbit
    • Label: R-PE
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: G-protein coupled receptor 65, T-cell death-associated gene 8 protein, Psychosine receptor
  • Anti-Human CXCR5 (extracellular) Antibody
    Anti-Human CXCR5 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CXCR5 Chemokine Receptor
    • Product Code: ACR-015
    • Short Description: A Rabbit Polyclonal Antibody to CXCR5 Chemokine Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: C-X-C chemokine receptor type 5, Burkitt lymphoma receptor 1, BLR1, Monocyte-derived receptor 15, MDR15, CD185
  • Anti-Human TNF Receptor I (extracellular) Antibody
    Anti-Human TNF Receptor I (extracellular) Antibody
    A Rabbit Polyclonal Antibody to TNF Receptor 1
    • Product Code: ANT-041
    • Short Description: A Rabbit Polyclonal Antibody to TNF Receptor 1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: Tumor necrosis factor receptor 1, TNFR1, TNFRSF1A, TNF receptor superfamily member 1A, CD120a
  • Anti-LINGO-1 (extracellular) Antibody
    Anti-LINGO-1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to LINGO-1
    • Product Code: ANT-032
    • Short Description: A Rabbit Polyclonal Antibody to LINGO-1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 1, Leucine-rich repeat neuronal protein 1, Leucine-rich repeat neuronal protein 6A, LRRN6A, LERN1
  • Anti-NMDAR2A (GluN2A) (extracellular) Antibody
    Anti-NMDAR2A (GluN2A) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to NMDA Receptor 2A (GluN2A)
    • Product Code: AGC-002
    • Short Description: A Rabbit Polyclonal Antibody to NMDA Receptor 2A (GluN2A)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: NMDA receptor 2A, NR2A, GRIN2A, Ionotropic glutamate receptor subunit ε1, N-methyl-D-aspartate receptor subunit 2A
  • Anti-NMDAR2A (GluN2A) (extracellular)-ATTO Fluor-488 Antibody
    Anti-NMDAR2A (GluN2A) (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to NMDA Receptor 2A (GluN2A) Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: AGC-002-AG
    • Short Description: A Rabbit Polyclonal Antibody to NMDA Receptor 2A (GluN2A) Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: NMDA receptor 2A, NR2A, GRIN2A, Ionotropic glutamate receptor subunit ε1, N-methyl-D-aspartate receptor subunit 2A
  • Anti-NMDAR2B (GluN2B) (extracellular) Antibody
    KO Validate'
    Anti-NMDAR2B (GluN2B) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to NMDA Receptor 2B (GluN2B)
    • Product Code: AGC-003
    • Short Description: A Rabbit Polyclonal Antibody to NMDA Receptor 2B (GluN2B)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: NMDA receptor 2B, Ionotropic glutamate receptor subunit ε2, N-methyl-D-aspartate receptor subunit 2B, GRIN2B, NR2B
  • Anti-NMDAR2B (GluN2B) (extracellular)-ATTO Fluor-594 Antibody
    Anti-NMDAR2B (GluN2B) (extracellular)-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to NMDA Receptor 2B (GluN2B) Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: AGC-003-AR
    • Short Description: A Rabbit Polyclonal Antibody to NMDA Receptor 2B (GluN2B) Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: NMDA receptor 2B, Ionotropic glutamate receptor subunit ε2, N-methyl-D-aspartate receptor subunit 2B, GRIN2B, NR2B
  • Anti-NMDAR2C (GRIN2C) (extracellular) Antibody
    Anti-NMDAR2C (GRIN2C) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to NMDA Receptor 2C (GRIN2C)
    • Product Code: AGC-018
    • Short Description: A Rabbit Polyclonal Antibody to NMDA Receptor 2C (GRIN2C)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: NMDA receptor 2C, NR2C, GluN2C, Ionotropic glutamate receptor subunit ε3, N-methyl-D-aspartate receptor subunit 2C
  • Anti-NMDAR2D (GRIN2D) (extracellular) Antibody
    Anti-NMDAR2D (GRIN2D) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to NMDA Receptor 2D (GRIN2D)
    • Product Code: AGC-020
    • Short Description: A Rabbit Polyclonal Antibody to NMDA Receptor 2D (GRIN2D)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: NMDA receptor 2D, NR2D, GluN2D, Ionotropic glutamate receptor subunit ε4, N-methyl-D-aspartate receptor subunit 2D
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