Neuroinflammation

Neuroinflammation

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

123 Results
  • Anti-Aquaporin 4 (AQP4) (300-314)-ATTO Fluor-594 Antibody
    Anti-Aquaporin 4 (AQP4) (300-314)-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to Aquaporin 4 Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: AQP-014-AR
    • Short Description: A Rabbit Polyclonal Antibody to Aquaporin 4 Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: WCH4, Mercurial-insensitive water channel
  • Anti-ASIC5 (extracellular) Antibody
    Anti-ASIC5 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to ASIC5 Channel
    • Product Code: ASC-032
    • Short Description: A Rabbit Polyclonal Antibody to ASIC5 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Acid-sensing ion channel 5, Amiloride-sensitive cation channel 5, Brain-liver-intestine amiloride-sensitive Na+ channel, BLINaC, HINAC, ACCN5
  • Anti-CCR2 (extracellular) Antibody
    Anti-CCR2 (extracellular) Antibody
    A Rabbit Polyclonal antibody to CCR2 (extracellular)
    • Product Code: ACR-072
    • Short Description: A Rabbit Polyclonal antibody to CCR2 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: C-C chemokine receptor type 2, Monocyte chemoattractant protein 1 receptor, MCP-1 receptor, CC-CKR-2, CD192
  • Anti-CCR2 (extracellular)-FITC Antibody
    Anti-CCR2 (extracellular)-FITC Antibody
    A Rabbit Polyclonal antibody to CCR2 (extracellular) conjugated to FITC fluorophore.
    • Product Code: ACR-072-F
    • Short Description: A Rabbit Polyclonal antibody to CCR2 (extracellular) conjugated to FITC fluorophore.
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: C-C chemokine receptor type 2, Monocyte chemoattractant protein 1 receptor, MCP-1 receptor, CC-CKR-2, CD192
  • Anti-CCR9 (extracellular) Antibody
    Anti-CCR9 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CCR9 Chemokine Receptor
    • Product Code: ACR-029
    • Short Description: A Rabbit Polyclonal Antibody to CCR9 Chemokine Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: C-C chemokine receptor type 9, CC-CKR-9, G-protein coupled receptor 28, GPR28, GPR-9-6, CDw199
  • Anti-GPR17 Antibody
    Anti-GPR17 Antibody
    A Rabbit Polyclonal Antibody to GPR17 Receptor
    • Product Code: AGR-048
    • Short Description: A Rabbit Polyclonal Antibody to GPR17 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: G-protein coupled receptor 17, Uracil nucleotide/cysteinyl leukotriene receptor, UDP/CysLT Receptor, P2Y-like receptor
  • Anti-GPR183 (extracellular) Antibody
    Anti-GPR183 (extracellular) Antibody
    A Rabbit Polyclonal antibody to GPR183 (extracellular).
    • Product Code: AGR-063
    • Short Description: A Rabbit Polyclonal antibody to GPR183 (extracellular).
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunohistochemistry
    • Target: G-Protein Coupled Receptor 183, Epstein-Barr Virus-Induced G-Protein Coupled Receptor 2, EBI2
  • Anti-GPR183 (extracellular)-FITC Antibody
    Anti-GPR183 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to GPR183 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: AGR-063-F
    • Short Description: A Rabbit Polyclonal Antibody to GPR183 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: G-Protein Coupled Receptor 183, Epstein-Barr Virus-Induced G-Protein Coupled Receptor 2, EBI2
  • Anti-GPR65 (TDAG8) (extracellular) Antibody
    Anti-GPR65 (TDAG8) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to GPR65 (TDAG8)
    • Product Code: AGR-043
    • Short Description: A Rabbit Polyclonal Antibody to GPR65 (TDAG8)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: G-protein coupled receptor 65, T-cell death-associated gene 8 protein, Psychosine receptor
  • Anti-GPR65 (TDAG8) (extracellular)-FITC Antibody
    Anti-GPR65 (TDAG8) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to GPR65 (TDAG8) Conjugated to the Fluorescent Dye FITC
    • Product Code: AGR-043-F
    • Short Description: A Rabbit Polyclonal Antibody to GPR65 (TDAG8) Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: G-protein coupled receptor 65, T-cell death-associated gene 8 protein, Psychosine receptor
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