Neuroinflammation

Neuroinflammation

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

123 Results
  • Anti-P2Y13 Receptor (extracellular) Antibody
    Anti-P2Y13 Receptor (extracellular) Antibody
    A Rabbit Polyclonal antibody to P2Y13 (extracellular).
    • Product Code: APR-103
    • Short Description: A Rabbit Polyclonal antibody to P2Y13 (extracellular).
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: Purinergic Receptor P2Y13, P2RY13, GPCR1, GPR86, GPR94
  • Anti-P2Y13 Receptor (extracellular)-FITC Antibody
    Anti-P2Y13 Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to P2Y13 Receptor  (extracellular) conjugated to the fluorescent dye FITC 
    • Product Code: APR-103-F
    • Short Description: A Rabbit Polyclonal Antibody to P2Y13 Receptor  (extracellular) conjugated to the fluorescent dye FITC 
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Purinergic Receptor P2Y13, P2RY13, GPCR1, GPR86, GPR94, SP174
  • Anti-P2Y13 Receptor Antibody
    KO Validate'
    Anti-P2Y13 Receptor Antibody
    A Rabbit Polyclonal Antibody to P2Y13 Receptor
    • Product Code: APR-017
    • Short Description: A Rabbit Polyclonal Antibody to P2Y13 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: P2Y purinoceptor 13, P2RY13, GPCR1, GPR86, GPR94
  • Anti-pan ASIC (extracellular) Antibody
    Anti-pan ASIC (extracellular) Antibody
    A Rabbit Polyclonal Antibody to ASIC Channels (ASIC1, ASIC2, ASIC3, ASIC4)
    • Product Code: ASC-031
    • Short Description: A Rabbit Polyclonal Antibody to ASIC Channels (ASIC1, ASIC2, ASIC3, ASIC4)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Acid-sensing ion channel, Acid-sensing (proton-gated) ion channel, ACCN, ACCN2, ACCN3, ACCN4
  • Anti-RGMA (extracellular) Antibody
    Anti-RGMA (extracellular) Antibody
    A Rabbit polyclonal antibody to RGMA (extracellular).
    • Product Code: ANR-126
    • Short Description: A Rabbit polyclonal antibody to RGMA (extracellular).
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunohistochemistry
    • Target: Repulsive Guidance Molecule A, RGM Domain Family Member A, Repulsive Guidance Molecule BMP Co-Receptor A, RGMa
  • Anti-S1PR1 (EDG1) (extracellular) Antibody
    Anti-S1PR1 (EDG1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 1
    • Product Code: ASR-011
    • Short Description: A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Sphingosine 1-phosphate receptor 1, S1P1, Endothelial differentiation G-protein coupled receptor 1, CD363, CHEDG1
  • Anti-S1PR1 (EDG1) (extracellular)-FITC Antibody
    Anti-S1PR1 (EDG1) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 1 Conjugated to the Fluorescent Dye FITC
    • Product Code: ASR-011-F
    • Short Description: A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 1 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Sphingosine 1-phosphate receptor 1, S1P1, Endothelial differentiation G-protein coupled receptor 1, CHEDG1, CD363
  • Anti-S1PR2 (EDG5) Antibody
    Anti-S1PR2 (EDG5) Antibody
    A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 2
    • Product Code: ASR-012
    • Short Description: A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Sphingosine 1-phosphate receptor 2, S1P2, Endothelial differentiation G-protein coupled receptor 5, Lysophospholipid receptor B2, LPB2
  • Anti-S1PR3 (EDG3) (extracellular) Antibody
    Anti-S1PR3 (EDG3) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 3
    • Product Code: ASR-013
    • Short Description: A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: Sphingosine 1-phosphate receptor 3, S1P3, Endothelial differentiation G-protein coupled receptor 3, Lysophospholipid receptor B3, LPB3
  • Anti-S1PR3 (EDG3) (extracellular)-ATTO Fluor-488 Antibody
    Anti-S1PR3 (EDG3) (extracellular)-ATTO Fluor-488 Antibody
    A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 3 Conjugated to the Fluorescent Dye ATTO-488
    • Product Code: ASR-013-AG
    • Short Description: A Rabbit Polyclonal Antibody to Sphingosine 1-Phosphate Receptor 3 Conjugated to the Fluorescent Dye ATTO-488
    • Host: Rabbit
    • Label: ATTO 488
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: Sphingosine 1-phosphate receptor 3, S1P3, Endothelial differentiation G-protein coupled receptor 3, Lysophospholipid receptor B3, LPB3
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