Neuroplasticity

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

377 Results
  • Anti-GPR171 (extracellular) Antibody
    Anti-GPR171 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to GPR171
    • Product Code: AGR-054
    • Short Description: A Rabbit Polyclonal Antibody to GPR171
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: G-Protein Coupled Receptor 171, Platelet Activating Receptor Homolog, G-protein coupled receptor H963
  • Anti-GPR171 (extracellular)-PE Antibody
    Anti-GPR171 (extracellular)-PE Antibody
    A Rabbit Polyclonal Antibody to GPR171 (extracellular) conjugated to the fluorescent dye Phycoerythrin (PE)
    • Product Code: AGR-054-PE
    • Short Description: A Rabbit Polyclonal Antibody to GPR171 (extracellular) conjugated to the fluorescent dye Phycoerythrin (PE)
    • Host: Rabbit
    • Label: R-PE
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: G-Protein Coupled Receptor 171, Platelet Activating Receptor Homolog, G-protein coupled receptor H963
  • Anti-GRIK3 (GluK3) (extracellular) Antibody
    Anti-GRIK3 (GluK3) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Kainate Receptor GluK3
    • Product Code: AGC-040
    • Short Description: A Rabbit Polyclonal Antibody to Kainate Receptor GluK3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Glutamate receptor ionotropic kainate 3, Glutamate receptor 7, GluR7
  • Anti-GRIK5 (GluK5) (extracellular) Antibody
    Anti-GRIK5 (GluK5) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Kainate Receptor GluK5
    • Product Code: AGC-042
    • Short Description: A Rabbit Polyclonal Antibody to Kainate Receptor GluK5
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Glutamate receptor ionotropic kainate 5, Glutamate receptor KA-2, KA2
  • Anti-GSG1L Antibody
    Anti-GSG1L Antibody
    A Rabbit Polyclonal Antibody to GSG1L
    • Product Code: AGC-048
    • Short Description: A Rabbit Polyclonal Antibody to GSG1L
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Germ cell-specific gene 1-like protein, GSG1-like protein
  • Anti-Human ZACN (extracellular) Antibody
    Anti-Human ZACN (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Zinc-Activated Ion Channel
    • Product Code: AZC-001
    • Short Description: A Rabbit Polyclonal Antibody to Zinc-Activated Ion Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: Zinc-activated ligand-gated ion channel, Ligand-gated ion channel zinc-activated 1, Ligand-gated ion-channel receptor L2, ZAC, LGICZ1
  • Anti-Kidins220 Antibody
    Anti-Kidins220 Antibody
    A Rabbit Polyclonal Antibody to Kidins220
    • Product Code: APZ-017
    • Short Description: A Rabbit Polyclonal Antibody to Kidins220
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Kinase D-interacting substrate of 220 kDa, Ankyrin repeat-rich membrane-spanning protein, ARMS
  • Anti-LRRC26 Antibody
    Anti-LRRC26 Antibody
    A Rabbit Polyclonal Antibody to LRRC26
    • Product Code: APC-070
    • Short Description: A Rabbit Polyclonal Antibody to LRRC26
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: Leucine-rich repeat-containing protein 26, BK channel auxiliary gamma subunit LRRC26, Cytokeratin-associated protein in cancer, CAPC
  • Anti-mGluR6 (extracellular) Antibody
    Anti-mGluR6 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 6
    • Product Code: AGC-026
    • Short Description: A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 6
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Metabotropic glutamate receptor 6, GRM6, mGlu6, Gprc1f
  • Anti-NALCN/VGCNL1 (extracellular) Antibody
    Anti-NALCN/VGCNL1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Sodium Leak Channel Non-Selective Protein (NALCN)
    • Product Code: ASC-022
    • Short Description: A Rabbit Polyclonal Antibody to Sodium Leak Channel Non-Selective Protein (NALCN)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Sodium leak channel non-selective protein, Voltage gated channel-like protein 1, Four domain-type voltage-gated ion channel alpha-1 subunit, CanIon, Rb21-channel
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