Neuroplasticity

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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

377 Results
  • Anhydroryanodine
    Anhydroryanodine
    A Non Active Analogue of Ryanodine
    • Product Code: A-510
    • Short Description: A Non Active Analogue of Ryanodine
    • CAS No.: 6883-31-4
    • MW: 475.54
  • Anisomycin
    Bioassay Tested
    Anisomycin
    Protein Synthesis Inhibitor
    • Product Code: A-520
    • Short Description: Protein Synthesis Inhibitor
    • CAS No.: 22862-76-6
    • MW: 265.3
  • Anti-5HT5B Receptor (HTR5B) (extracellular) Antibody
    Anti-5HT5B Receptor (HTR5B) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 5B
    • Product Code: ASR-040
    • Short Description: A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 5B
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: 5-Hydroxytryptamine receptor 5B, Serotonin receptor 5B, MR22
  • Anti-ASIC4 Antibody
    Anti-ASIC4 Antibody
    A Rabbit Polyclonal Antibody to ASIC4 Channel
    • Product Code: ASC-015
    • Short Description: A Rabbit Polyclonal Antibody to ASIC4 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Acid-sensing ion channel 4, H+-gated cation channel, Amiloride-sensitive cation channel 4, BNaC4, SPASIC, ACCN4
  • Anti-Beta-Synuclein Antibody
    Anti-Beta-Synuclein Antibody
    A Rabbit Polyclonal Antibody to β-Synuclein
    • Product Code: APZ-036
    • Short Description: A Rabbit Polyclonal Antibody to β-Synuclein
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: β-Synuclein, Synuclein beta, PNP 14, SNCB
  • Anti-CaBP1 Antibody
    Anti-CaBP1 Antibody
    A Rabbit Polyclonal Antibody to Calcium-Binding Protein 1
    • Product Code: ACS-002
    • Short Description: A Rabbit Polyclonal Antibody to Calcium-Binding Protein 1
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: Calcium-binding protein 1, Caldendrin, Calbrain
  • Anti-CHERP Antibody
    Anti-CHERP Antibody
    A Rabbit Polyclonal Antibody to CHERP
    • Product Code: ACC-330
    • Short Description: A Rabbit Polyclonal Antibody to CHERP
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Calcium homeostasis endoplasmic reticulum protein, SR-related CTD-associated factor 6, SCAF6
  • Anti-CHERP-ATTO Fluor-594 Antibody
    Anti-CHERP-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to CHERP Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ACC-330-AR
    • Short Description: A Rabbit Polyclonal Antibody to CHERP Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Calcium homeostasis endoplasmic reticulum protein, SR-related CTD-associated factor 6, SCAF6
  • Anti-Choline Transporter (SLC5A7) (extracellular) Antibody
    Anti-Choline Transporter (SLC5A7) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Choline Transporter (SLC5A7)
    • Product Code: ACT-001
    • Short Description: A Rabbit Polyclonal Antibody to Choline Transporter (SLC5A7)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Na+/Cl--Dependent high affinity choline transporter 1, Hemicholinium-3-sensitive choline transporter, CHT, CHT1
  • Anti-CNIH2/3 (extracellular) Antibody
    Anti-CNIH2/3 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to CNIH2 and CNIH3
    • Product Code: AGC-047
    • Short Description: A Rabbit Polyclonal Antibody to CNIH2 and CNIH3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: Cornichon homolog 2, Cornichon homolog 3, Cornichon family AMPA receptor auxiliary protein 2, Cornichon family AMPA receptor auxiliary protein 3
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