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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

771 Results
  • Anti-Human Na<sub>V</sub>1.7 (SCN9A) (extracellular) Antibody
    Anti-Human NaV1.7 (SCN9A) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Human NaV1.7 (SCN9A)
    • Product Code: ASC-029
    • Short Description: A Rabbit Polyclonal Antibody to Human NaV1.7 (SCN9A)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry
    • Target: Voltage-gated sodium channel type IX subunit alpha, PN1
  • Anti-Human P2Y11 Receptor Antibody
    Anti-Human P2Y11 Receptor Antibody
    A Rabbit Polyclonal Antibody to P2Y11 Receptor
    • Product Code: APR-015
    • Short Description: A Rabbit Polyclonal Antibody to P2Y11 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
    • Target: P2Y purinoceptor 11, P2RY11
  • Anti-Human TRPV1 (extracellular) Antibody
    Anti-Human TRPV1 (extracellular) Antibody
    A Rabbit Polyclonal antibody to Human TRPV1 (extracellular)
    • Product Code: ACC-334
    • Short Description: A Rabbit Polyclonal antibody to Human TRPV1 (extracellular)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: Transient receptor potential cation channel subfamily V member 1, Capsaicin receptor, Vanilloid receptor 1, VR1, OTRPC1
  • Anti-Human TRPV1 (extracellular)-APC Antibody
    Anti-Human TRPV1 (extracellular)-APC Antibody
    A Rabbit Polyclonal Antibody to Human TRPV1 (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Product Code: ACC-334-APC
    • Short Description: A Rabbit Polyclonal Antibody to Human TRPV1 (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Host: Rabbit
    • Label: APC
    • Reactivity: Human
    • Applications: Flow cytometry
    • Target: Transient receptor potential cation channel subfamily V member 1, Capsaicin receptor, Vanilloid receptor 1, VR1, OTRPC1
  • Anti-Human TRPV1 (extracellular)-FITC Antibody
    Anti-Human TRPV1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody Human TRPV1 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ACC-334-F
    • Short Description: A Rabbit Polyclonal Antibody Human TRPV1 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human
    • Applications: Live cell imaging, Flow cytometry
    • Target: Transient receptor potential cation channel subfamily V member 1, Capsaicin receptor, Vanilloid receptor 1, VR1, OTRPC1
  • Anti-Human TRPV2 (VRL1) (extracellular) Antibody
    Anti-Human TRPV2 (VRL1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to TRPV2
    • Product Code: ACC-139
    • Short Description: A Rabbit Polyclonal Antibody to TRPV2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot
    • Target: Vanilloid receptor-like protein 1, VRL-1, Osm-9-like TRP channel 2, OTRPC2, Strech-activated channel 2B, SAC2B
  • Anti-Human TRPV2 (VRL1) (extracellular)-FITC Antibody
    Anti-Human TRPV2 (VRL1) (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Human TRPV2 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ACC-139-F
    • Short Description: A Rabbit Polyclonal Antibody to Human TRPV2 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human
    • Applications: Live cell imaging, Flow cytometry
    • Target: Vanilloid receptor-like protein 1, VRL-1, Osm-9-like TRP channel 2, OTRPC2
  • Anti-Human ZACN (extracellular) Antibody
    Anti-Human ZACN (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Zinc-Activated Ion Channel
    • Product Code: AZC-001
    • Short Description: A Rabbit Polyclonal Antibody to Zinc-Activated Ion Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: Zinc-activated ligand-gated ion channel, Ligand-gated ion channel zinc-activated 1, Ligand-gated ion-channel receptor L2, ZAC, LGICZ1
  • Anti-KChIP1 (KCNIP1) Antibody
    Anti-KChIP1 (KCNIP1) Antibody
    A Rabbit Polyclonal Antibody to KChIP1 Subunit
    • Product Code: APC-141
    • Short Description: A Rabbit Polyclonal Antibody to KChIP1 Subunit
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot
    • Target: KV channel-interacting protein 1, VABP, Vesicle APC-binding protein, A-type potassium channel modulatory protein 1, Potassium channel-interacting protein 1
  • Anti-KChIP2 Antibody
    Anti-KChIP2 Antibody
    A Rabbit Polyclonal Antibody to KChIP2 Subunit
    • Product Code: APC-142
    • Short Description: A Rabbit Polyclonal Antibody to KChIP2 Subunit
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: KCNIP2, KV channel-interacting protein 2, A-type potassium channel modulatory protein 2, Cardiac voltage-gated potassium channel modulatory subunit
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