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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

771 Results
  • Anti-P2Y6 Receptor (extracellular)-FITC Antibody
    Anti-P2Y6 Receptor (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to P2Y6 Conjugated to the Fluorescent Dye FITC
    • Product Code: APR-106-F
    • Short Description: A Rabbit Polyclonal Antibody to P2Y6 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: P2Y purinoceptor 6, P2RY6
  • Anti-P2Y6 Receptor (extracellular)-PE Antibody
    Anti-P2Y6 Receptor (extracellular)-PE Antibody
    A Rabbit Polyclonal Antibody to P2Y6 Receptor (extracellular) conjugated to the fluorescent dye R-Phycoerythrin (R-PE)
    • Product Code: APR-106-PE
    • Short Description: A Rabbit Polyclonal Antibody to P2Y6 Receptor (extracellular) conjugated to the fluorescent dye R-Phycoerythrin (R-PE)
    • Host: Rabbit
    • Label: R-PE
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: P2Y purinoceptor 6, P2RY6
  • Anti-P2Y6 Receptor Antibody
    KO Validate'
    Anti-P2Y6 Receptor Antibody
    A Rabbit Polyclonal Antibody to P2Y6 Receptor
    • Product Code: APR-011
    • Short Description: A Rabbit Polyclonal Antibody to P2Y6 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: P2Y purinoceptor 6, P2RY6
  • Anti-pan ASIC (extracellular) Antibody
    Anti-pan ASIC (extracellular) Antibody
    A Rabbit Polyclonal Antibody to ASIC Channels (ASIC1, ASIC2, ASIC3, ASIC4)
    • Product Code: ASC-031
    • Short Description: A Rabbit Polyclonal Antibody to ASIC Channels (ASIC1, ASIC2, ASIC3, ASIC4)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Acid-sensing ion channel, Acid-sensing (proton-gated) ion channel, ACCN, ACCN2, ACCN3, ACCN4
  • Anti-Pan Na<sub>V</sub> Antibody
    Anti-Pan NaV Antibody
    A Rabbit Polyclonal Antibody to Pan NaV Channel
    • Product Code: ASC-003
    • Short Description: A Rabbit Polyclonal Antibody to Pan NaV Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Pan sodium channel, SP19
  • Anti-Pannexin 2 (extracellular) Antibody
    Anti-Pannexin 2 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Pannexin 2
    • Product Code: ACC-232
    • Short Description: A Rabbit Polyclonal Antibody to Pannexin 2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: PANX2, PX2, hPANX2
  • Anti-Piezo1 (extracellular) Antibody
    KO Validate'
    Anti-Piezo1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Piezo1 Channel
    • Product Code: APC-087
    • Short Description: A Rabbit Polyclonal Antibody to Piezo1 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Piezo-type mechanosensitive ion channel component 1, Protein FAM38A, Mib, Membrane protein induced by beta-amyloid treatment
  • Anti-Piezo1 (extracellular)-APC Antibody
    Anti-Piezo1 (extracellular)-APC Antibody
    A Rabbit Polyclonal Antibody to Piezo1 (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Product Code: APC-087-APC
    • Short Description: A Rabbit Polyclonal Antibody to Piezo1 (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)
    • Host: Rabbit
    • Label: APC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Piezo-type mechanosensitive ion channel component 1, Protein FAM38A, Mib, Membrane protein induced by beta-amyloid treatment
  • Anti-Piezo1 (extracellular)-FITC Antibody
    Anti-Piezo1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to Piezo1 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: APC-087-F
    • Short Description: A Rabbit Polyclonal Antibody to Piezo1 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: Piezo-type mechanosensitive ion channel component 1, Protein FAM38A, Mib, Membrane protein induced by beta-amyloid treatment
  • Anti-Piezo2 Antibody
    KO Validate'
    Anti-Piezo2 Antibody
    A Rabbit Polyclonal Antibody to Piezo2
    • Product Code: APC-090
    • Short Description: A Rabbit Polyclonal Antibody to Piezo2
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunohistochemistry
    • Target: Piezo-Type Mechanosensitive Ion Channel Component 2, Protein FAM38B
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