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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

771 Results
  • Anti-TROY (TNFRSF19) (extracellular) Antibody
    Anti-TROY (TNFRSF19) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to TROY
    • Product Code: ANT-033
    • Short Description: A Rabbit Polyclonal Antibody to TROY
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence
    • Target: TNF receptor superfamily member 19, Toxicity and JNK inducer, TAJ, TRADE
  • Anti-TRPA1 (extracellular) Antibody
    KO Validate'
    Anti-TRPA1 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to TRPA1 Channel
    • Product Code: ACC-037
    • Short Description: A Rabbit Polyclonal Antibody to TRPA1 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: Transient receptor potential cation channel subfamily A member 1, ANKTM1, Ankyrin-like with transmembrane domains protein 1, Transformation-sensitive protein p120
  • Anti-TRPA1 (extracellular)-FITC Antibody
    Anti-TRPA1 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to TRPA1 (extracellular) conjugated to the fluorescent dye FITC
    • Product Code: ACC-037-F
    • Short Description: A Rabbit Polyclonal Antibody to TRPA1 (extracellular) conjugated to the fluorescent dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Flow cytometry
    • Target: Transient receptor potential cation channel subfamily A member 1, ANKTM1, Ankyrin-like with transmembrane domains protein 1, Transformation-sensitive protein p120, Wasabi Receptor
  • Anti-TRPC2 Antibody
    Anti-TRPC2 Antibody
    A Rabbit Polyclonal Antibody to TRPC2 Channel
    • Product Code: ACC-027
    • Short Description: A Rabbit Polyclonal Antibody to TRPC2 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: Short transient receptor potential channel 2, Transient receptor protein 2, TRP2, mTrp2
  • Anti-TRPM3 (extracellular) Antibody
    Anti-TRPM3 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to TRPM3 Channel
    • Product Code: ACC-050
    • Short Description: A Rabbit Polyclonal Antibody to TRPM3 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Transient receptor potential cation channel subfamily M member 3, Long transient receptor potential channel 3, LTrpC3, Melastatin-2, MLSN2
  • Anti-TRPM8 (extracellular) Antibody
    KO Validate'
    Anti-TRPM8 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to TRPM8 Channel
    • Product Code: ACC-049
    • Short Description: A Rabbit Polyclonal Antibody to TRPM8 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Neutralization, Immunohistochemistry
    • Target: TRPP8, LTrpC6, Transient receptor potential cation channel subfamily M member 8
  • Anti-TRPML2 (Mucolipin-2) Antibody
    KO Validate'
    Anti-TRPML2 (Mucolipin-2) Antibody
    A Rabbit Polyclonal Antibody to TRPML2 (Mucolipin-2) Channel
    • Product Code: ACC-082
    • Short Description: A Rabbit Polyclonal Antibody to TRPML2 (Mucolipin-2) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Mcoln2
  • Anti-TRPML3 (Mucolipin 3) Antibody
    Anti-TRPML3 (Mucolipin 3) Antibody
    A Rabbit Polyclonal Antibody to TRPML3 (Mucolipin 3) Channel
    • Product Code: ACC-083
    • Short Description: A Rabbit Polyclonal Antibody to TRPML3 (Mucolipin 3) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Mcoln3
  • Anti-TRPML3 (Mucolipin 3)-ATTO Fluor-594 Antibody
    Anti-TRPML3 (Mucolipin 3)-ATTO Fluor-594 Antibody
    A Rabbit Polyclonal Antibody to TRPML3 Channel Conjugated to the Fluorescent Dye ATTO-594
    • Product Code: ACC-083-AR
    • Short Description: A Rabbit Polyclonal Antibody to TRPML3 Channel Conjugated to the Fluorescent Dye ATTO-594
    • Host: Rabbit
    • Label: ATTO 594
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: Mcoln3
  • Anti-TRPV1 (VR1) (extracellular) Antibody
    Anti-TRPV1 (VR1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to Rat TRPV1 (VR1) Channel
    • Product Code: ACC-029
    • Short Description: A Rabbit Polyclonal Antibody to Rat TRPV1 (VR1) Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Transient receptor potential cation channel subfamily V member 1, Capsaicin receptor, Vanilloid receptor 1, OTRPC1
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