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  • The expected molecular weight (MW) listed in the product datasheet is based solely on the size of the target protein’s amino acid sequence. It’s important to remember that many factors can affect the banding pattern of your western blot including: (1) the existence of a splice variant, (2) the quality of the loaded sample, (3) the protein extraction method, and (4) the protein transfer conditions. To achieve accurate results, you may need to systematically adjust the protein extraction method or the protein transfer conditions.

    • If the band’s MW is below the expected MW it could be due to a splice variant with a slightly different MW.
      • Heat the samples at 70°C for 10 min.
      • Increase the transfer time.
    • If the band’s MW is above the expected MW, it could be due to post-translational modifications.
    • In either event, you should use a blocking peptide as a negative control.

  • Some toxins and peptides are soluble in DMSO; you can find this information in the product specification section. For these products, prepare a concentrated stock solution first.

    1. Centrifuge vial (10,000 x g, 5 minutes) before adding solvent.
    2. Dissolve the lyophilized reagent in DMSO. We recommend keeping a concentrated stock solution at 1-10 mM (100-1000X higher than the final working concentration).
    3. Once the peptide is completely dissolved in DMSO, slowly dilute the peptide in pure water (or buffer) to the desired final working concentration.

    Note: We recommend that you maintain a DMSO concentration as low as possible. For cell assays, a final concentration of 0.1–0.5% DMSO (v/v) is considered acceptable. For other experiments, 5% DMSO (v/v) is recommended; adjust this according to your experimental requirements.

  • FAQ 3FAQ 3FAQ 3FAQ 3

771 Results
  • Anti-TRPV4 (extracellular) Antibody
    Anti-TRPV4 (extracellular) Antibody
    A Rabbit Polyclonal Antibody to TRPV4 Channel
    • Product Code: ACC-124
    • Short Description: A Rabbit Polyclonal Antibody to TRPV4 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: OTRPC4, VRL-2, VR-OAC, TRP12
  • Anti-TRPV4 (extracellular) Antibody- PE Antibody
    Anti-TRPV4 (extracellular) Antibody- PE Antibody
    A Rabbit Polyclonal Antibody to TRPV4 (extracellular) conjugated to the fluorescent dye R-Phycoerythrin
    • Product Code: ACC-124-PE
    • Short Description: A Rabbit Polyclonal Antibody to TRPV4 (extracellular) conjugated to the fluorescent dye R-Phycoerythrin
    • Host: Rabbit
    • Label: R-PE
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: OTRPC4, VRL-2, VR-OAC, TRP12, Osm-9-Like TRP Channel 4
  • Anti-TRPV4 (extracellular)-FITC Antibody
    Anti-TRPV4 (extracellular)-FITC Antibody
    A Rabbit Polyclonal Antibody to TRPV4 Conjugated to the Fluorescent Dye FITC
    • Product Code: ACC-124-F
    • Short Description: A Rabbit Polyclonal Antibody to TRPV4 Conjugated to the Fluorescent Dye FITC
    • Host: Rabbit
    • Label: FITC
    • Reactivity: Human, Rat, Mouse
    • Applications: Live cell imaging, Flow cytometry
    • Target: OTRPC4, VRL-2, VR-OAC, TRP12, Osm-9-Like TRP Channel 4
  • Anti-TRPV4 Antibody
    KO Validate'
    Anti-TRPV4 Antibody
    A Rabbit Polyclonal Antibody to TRPV4 Channel
    • Product Code: ACC-034
    • Short Description: A Rabbit Polyclonal Antibody to TRPV4 Channel
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Western blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
    • Target: OTRPC4, VRL-2, VR-OAC, TRP12
  • Anti-TRPV4-ATTO Fluor-550 Antibody
    KO Validate'
    Anti-TRPV4-ATTO Fluor-550 Antibody
    A Rabbit Polyclonal Antibody to TRPV4 Channel Conjugated to the Fluorescent Dye ATTO-550
    • Product Code: ACC-034-AO
    • Short Description: A Rabbit Polyclonal Antibody to TRPV4 Channel Conjugated to the Fluorescent Dye ATTO-550
    • Host: Rabbit
    • Label: ATTO 550
    • Reactivity: Human, Rat, Mouse
    • Applications: Immunofluorescence, Immunohistochemistry
    • Target: OTRPC4, VRL-2, VR-OAC, TRP12
  • Anti-Vasopressin V<sub>1B</sub> Receptor (AVPR1B) Antibody
    Anti-Vasopressin V1B Receptor (AVPR1B) Antibody
    A Rabbit Polyclonal Antibody to Vasopressin V1B Receptor
    • Product Code: AVR-011
    • Short Description: A Rabbit Polyclonal Antibody to Vasopressin V1B Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: V1bR, AVPR V1b, AVPR V3, Antidiuretic hormone receptor 1b, Arginine vasopressin receptor 1B, Vasopressin V3 receptor
  • Anti-Vasopressin V<sub>2</sub> Receptor (AVPR2) Antibody
    Anti-Vasopressin V2 Receptor (AVPR2) Antibody
    A Rabbit Polyclonal Antibody to Vasopressin V2 Receptor
    • Product Code: AVR-012
    • Short Description: A Rabbit Polyclonal Antibody to Vasopressin V2 Receptor
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot
    • Target: V2R, AVPR V2, Antidiuretic hormone receptor, ADHR, Renal-type arginine vasopressin receptor, DIR3, Nephrogenic diabetes insipidus
  • Anti-Vesicular Acetylcholine Transporter (VAChT) Antibody
    Anti-Vesicular Acetylcholine Transporter (VAChT) Antibody
    A Rabbit Polyclonal Antibody to Vesicular Acetylcholine Transporter
    • Product Code: ACT-003
    • Short Description: A Rabbit Polyclonal Antibody to Vesicular Acetylcholine Transporter
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Solute carrier family 18 member 3, SLC18A3
  • Anti-VGLUT3 Antibody
    Anti-VGLUT3 Antibody
    A Rabbit Polyclonal Antibody to Vesicular Glutamate Transporter 3
    • Product Code: AGC-037
    • Short Description: A Rabbit Polyclonal Antibody to Vesicular Glutamate Transporter 3
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Rat, Mouse
    • Applications: Western blot, Immunofluorescence, Immunohistochemistry
    • Target: Vesicular glutamate transporter 3, SLC17A8
  • Anti-µ-Opioid Receptor (OPRM1) (extracellular) Antibody
    Anti-µ-Opioid Receptor (OPRM1) (extracellular) Antibody
    A Rabbit Polyclonal Antibody to µ-Opioid Receptor (OPRM1)
    • Product Code: AOR-011
    • Short Description: A Rabbit Polyclonal Antibody to µ-Opioid Receptor (OPRM1)
    • Host: Rabbit
    • Label: Unconjugated
    • Reactivity: Human, Rat, Mouse
    • Applications: Indirect flow cytometry, Live cell imaging, Western blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
    • Target: Mu-type opioid receptor, Opioid receptor B, MOR-1, MOP, Mu-opiate receptor
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